Processing kodak motion picture films, module 3 analytical procedures

Regression
The data collected may be used to construct the regression
equation for the calibration line.
The reliability of the calibration line generated was done
in the following manner on a SHIMADZU Model UV16OU
Spectrophotometer. Four “fresh” ECN-2 and ECP-2D,
“KUL” bleach solutions were prepared containing 0.2515,
1.0235, 2.0296, and 3.0401 g/L iron(II). Each solution was
analyzed in triplicate to create a linear regression (based on
12 data points). The average standard deviation (1s) for the
linear regression was 0.007 g/L iron(II), corresponding to a
95 percent confidence estimate of ± 0.015 g/L. Each
laboratory should calibrate its spectrophotometer; otherwise,
an unknown bias may exist.
Process ECN-2
g wt of Fe(NH 4) 2(SO 4) 2 6H 2O g/L Iron(II) Absorbance
at 510 nm
Process ECP-2D
0.0887 0.2520 0.117
0.0887 0.2520 0.114
0.0887 0.2520 0.117
0.3562 1.0121 0.422
0.3562 1.0121 0.420
0.3562 1.0121 0.422
0.7101 2.0177 0.835
0.7101 2.0177 0.821
0.7101 2.0177 0.839
1.0658 3.0381 1.239
1.0658 3.0381 1.231
1.0658 3.0381 1.234
g wt of Fe(NH 4 ) 2 (SO 4 ) 2 6H 2 O g/L Iron(II) Absorbance
at 510 nm
0.0885 0.2515 0.132
0.0885 0.2515 0.129
0.0885 0.2515 0.132
0.3602 1.0235 0.435
0.3602 1.0235 0.435
0.3602 1.0235 0.435
0.7143 2.0296 0.844
0.7143 2.0296 0.842
0.7143 2.0296 0.840
1.0665 3.0401 1.250
1.0665 3.0401 1.245
1.0665 3.0401 1.255
Figure 1
Calibration of Iron(II) in ECN-2, “KUL” Bleach
0
0
F002_0936AC
ABS. @ 510 nm
Figure 2
Calibration of Iron(II) in ECP-2D, “KUL” Bleach
This data was then processed by a least squares linear
regression to develop the line, y = mx + b.
where:
y = grams per litre of iron(II)
m = Slope of the line or the relation between
absorbance and iron(II) concentration determined
during calibration [(g/L)/absorbance]
x = absorbance of sample at 510 nm
b = the calibration intercept of the line with the y-axis
(g/L, Fe(II))
The equation generated using the above data was:
iron(II), g/L = 2.49 (A 510 ) – 0.068
y = 2.4889x - 0.0677
R2 = 0.9999
4 Processing KODAK Motion Picture Films, Module 3, Analytical Procedures H24.03
3.5
3
2.5
2
1.5
1
0.5
3.5
3
2.5
2
1.5
1
0.5
0
0
F002_0935AC
0.2
0.4
0.6
0.8
ABS. @ 510 nm
1
1.2
y = 2.4889x - 0.0677
R2 = 0.9999
0.2 0.4 0.6 0.8 1 1.2
1.4
1.4