Processing kodak motion picture films, module 3 analytical procedures

APPARATUS
Pipet (40-mL)
Graduated Cylinder (100 mL)
(2) Beakers (150 mL)
Conical Flask (250-mL)
Filter apparatus
All volumetric glassware should meet all Class A
specifications, as defined by American Society for Testing
and Materials (ASTM) Standards E 287, E288, and E969,
unless otherwise stated.
REAGENTS
All reagents should be ACS Reagent Grade unless otherwise
specified.
2.5 N Sodium hydroxide, NaOH
Concentrated Hydrochloric acid, HCl
Ferrous/Ferric Reagent
Water, Type I Reagent - This method was developed using
reagent water equivalent to or purer than Type I grade, as
defined in ASTM Standard D 1193. Other grades of
water, e.g., reverse osmosis (RO), demineralized, or
distilled water, may give equivalent results, but the effects
of water quality on method performance have not been
studied.
PROCEDURE
1. Pipet (wipe the pipet before leveling) 25.0 mL of
effluent sample (Solution A) into a 250 mL volumetric
flask. Dilute to volume with reagent water; stopper and
invert the flask several times to mix (this is
solution B).
2. Add, from a graduated cylinder, 100 mL of the diluted
sample (Solution B) from step 1 to a 250 mL conical
flask, and make it alkaline by adding 10 drops of 2.5 N
sodium hydroxide.
3. Mix thoroughly and filter the solution through
Whatman 2V filter paper.
4. Make the filtrate acid by the dropwise addition of
concentrated hydrochloric acid. (Use 0-14 pH
indicating paper as an indicator. The paper will turn
red when solution is acidic.)
5. Pipet (wipe the pipet before leveling) 40.0 mL of the
filtrate into each of two 150 mL beakers. (One will be
the sample and the other will be the blank.)
6. Add 2 drops of ferrous/ferric reagent to the first beaker
(sample); allow both beakers to stand for 15 minutes.
7. If a blue color is apparent, rinse and fill a 1-cm silica
cell with solution from the first 150 mL beaker
(step 6). Rinse the outer faces of the silica cell with
reagent water and wipe dry with a clean tissue.
Measure the absorbance of the sample vs. air at
700 nm on a spectrophotometer equipped with a
tungsten lamp. Record this reading as A700 spl.
Note: If no blue color is produced by Solution B in the
first beaker in Step 6, repeat Steps 2 through 8 using
the undiluted effluent sample (Solution A).
8. Rinse and fill the 1-cm silica cell from step 7 with the
filtrate in the second 150 mL beaker from step 5.
Measure the absorbance of this solution as in step 7.
Record this reading as A700 blk.
Processing KODAK Motion Picture Films, Module 3, Analytical Procedures H24.03 3