Processing kodak motion picture films, module 3 analytical procedures

APPENDIX A
Calibration of Spectrophotometer RA-1 in Reversal
Color Developer
This Appendix should be used to establish the initial
calibration equation, whenever the instrument has been
adjusted, or to recheck the calibration (at least every
six months)
A. Preparation of Standards
A stock standard solution without RA-1 is prepared by
dissolving the remaining required constituents of a Reversal
Color Developer in a 1 liter volumetric flask and diluting to
the mark with reagent water at room temperature.
1. The stock standard solution is added to a 100 mL
volumetric flask and labeled 0.0 mg/L RA-1.
2. Two mg of RA-1 is weighed to the nearest 0.1 mg and
added to a 100 mL volumetric flask and diluted to
volume with the stock standard solution. Stopper the
flask and invert six times to insure proper mixing. This
flask is labeled 2 mg/L RA-1.
3. Five mg of RA-1 is weighed to the nearest 0.1 mg and
added to a second 100 mL volumetric flask and diluted
to volume with the stock standard solution. Stopper the
flask and invert six times to insure proper mixing. This
flask is labeled 5 mg/L RA-1.
4. Seven mg of RA-1 is weighed to the nearest 0.1 mg
and added to a second 100 mL volumetric flask and
diluted to volume with the stock standard solution.
Stopper the flask and invert six times to insure proper
mixing. This flask is labeled 7 mg/L RA-1.
5. Ten mg of RA-1 is weighed to the nearest 0.1 mg and
added to a second 100 mL volumetric flask and diluted
to volume with the stock standard solution. Stopper the
flask and invert six times to insure proper mixing. This
flask is labeled 10 mg/L RA-1.
B. Analysis of Standards
1. Run each sample by the method described in the
preceding Procedure.
2. Table of Data gathered from Analysis of Standards
RA-1 Standard (mg/L) Abs 725 Abs 725
C. Regression
1. This data was processed by a least squares linear
regression to develop the line represented by equation,
y=mx+b:
Where:
y = mg/L RA-1
0.0 0.050 0.055
2.4 0.182 0.179
4.9 0.379 0.365
7.0 0.499 0.503
10.9 0.817 0.792
m = slope of the line or the relation between absorbance
and concentration determined during calibration
[(mg/L)/absorbance]
x = absorbance of sample at 725 nm
b = the intercept of the calibration line with the y-axis
(inmg/LRA-1)
2. The equation generated using the above data was:
RA-1, mg/L = 143.3 (ABS @725 nm ) - 4.355
3. The calibration equation was done in the following
manner on a (SHIMADZU Model UV 160 U)
spectrophotometer. Five fresh solutions were prepared
(see Step #1 of A. Preparation of Standards). Each
solution was analyzed twice to create a linear
regression, based on 10 data points, for each
spectrophotometer being used. Each laboratory should
calibrate their spectrophotometer, otherwise an
unknown bias may exist.
4 Processing KODAK Motion Picture Films, Module 3, Analytical Procedures H24.03