changes in protein profiles in bortezomib applied multiple myeloma ...

pH 3 IEF pH 10 pH 3 IEF pH 10
S
D
S
P
A
G
E
1 2 3
Figure 4.4. 2D PAGE Maps of Bortezomib (17 nM) and Control Group
In order to elucidate and compare protein profiles of two groups at issue, two
individual gel images were imported into gel analysis software DECODON Delta2D
Version 4.3. With the help of this software, these 2D PAGE gels were superposed and
comparative detailed analysis was performed. According to the analysis, 37 protein
spots were differentially expressed. Among them, 5 proteins were newly formed, 10
proteins lost, 12 proteins were up-regulated and 10 proteins were down-regulated as
compared to control group (untreated cells) (Figure 4.4). These spots were numbered
from 1 to 37, colored and labeled with a special name:
Newly formed proteins were marked with the green color (Y)
Lost proteins were marked with the red color (K)
Upregulated proteins were marked with the blue color (M)
Downregulated proteins were marked with the black color (S).
To observe differential expressed spots easily, gels were segmented and
magnified (Table 4.1).
4
9 15 16
10
5
11 12
14
13
20 22
17
24
37
36
19
35
21 25
23
34 28
26
33
32 29
31
30
6
27
7
18
8
Control
S
D
S
P
A
G
E
Bortezomib
71