changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
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pH 3 IEF pH 10 pH 3 IEF pH 10<br />
S<br />
D<br />
S<br />
P<br />
A<br />
G<br />
E<br />
1 2 3<br />
Figure 4.4. 2D PAGE Maps of Bortezomib (17 nM) and Control Group<br />
In order to elucidate and compare prote<strong>in</strong> <strong>profiles</strong> of two groups at issue, two<br />
<strong>in</strong>dividual gel images were imported <strong>in</strong>to gel analysis software DECODON Delta2D<br />
Version 4.3. With the help of this software, these 2D PAGE gels were superposed and<br />
comparative detailed analysis was performed. Accord<strong>in</strong>g to the analysis, 37 prote<strong>in</strong><br />
spots were differentially expressed. Among them, 5 prote<strong>in</strong>s were newly formed, 10<br />
prote<strong>in</strong>s lost, 12 prote<strong>in</strong>s were up-regulated and 10 prote<strong>in</strong>s were down-regulated as<br />
compared to control group (untreated cells) (Figure 4.4). These spots were numbered<br />
from 1 to 37, colored and labeled with a special name:<br />
Newly formed prote<strong>in</strong>s were marked with the green color (Y)<br />
Lost prote<strong>in</strong>s were marked with the red color (K)<br />
Upregulated prote<strong>in</strong>s were marked with the blue color (M)<br />
Downregulated prote<strong>in</strong>s were marked with the black color (S).<br />
To observe differential expressed spots easily, gels were segmented and<br />
magnified (Table 4.1).<br />
4<br />
9 15 16<br />
10<br />
5<br />
11 12<br />
14<br />
13<br />
20 22<br />
17<br />
24<br />
37<br />
36<br />
19<br />
35<br />
21 25<br />
23<br />
34 28<br />
26<br />
33<br />
32 29<br />
31<br />
30<br />
6<br />
27<br />
7<br />
18<br />
8<br />
Control<br />
S<br />
D<br />
S<br />
P<br />
A<br />
G<br />
E<br />
Bortezomib<br />
71