changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
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3.2.9.1. Isoelectric Focus<strong>in</strong>g (IEF) ................................................ 58<br />
3.2.9.2. SDS-PAGE ....................................................................... 61<br />
3.2.9.3. Sta<strong>in</strong><strong>in</strong>g of the Gel ............................................................ 63<br />
3.2.9.4. Image and Data Analysis of Gel ....................................... 64<br />
3.2.9.5. In-Gel Digestion ............................................................... 64<br />
3.2.9.6. ZipTip ............................................................................... 67<br />
3.2.9.7. Prote<strong>in</strong> Identification and Mass Spectrometric Analysis .. 67<br />
CHAPTER 4. RESULTS AND DISCUSSION .............................................................. 68<br />
4.1. Cytotoxic Effects of Bortezomib on MM U-266 Cells ...................... 68<br />
4.2. Changes <strong>in</strong> Caspase-3 Enzyme Activity and Loss of Mithocondrial<br />
Membrane Potantial (MMP)............................................................... 69<br />
4.3. Two-Dimensional Polyacrylamide Gel Electrophoresis Results........ 70<br />
CHAPTER 5. CONCLUSIONS ..................................................................................... 87<br />
REFERENCES ............................................................................................................... 89<br />
APPENDICES<br />
APPENDIX A. MEDIAS ............................................................................................. 102<br />
APPENDIX B. CHEMICALS, REAGENTS AND SOLUTIONS .............................. 103<br />
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