changes in protein profiles in bortezomib applied multiple myeloma ...

Table 3.4.
Table 3.3. Required Protein Sample with Rehydration Buffer
Strip Length 17 cm (pH 3 - 10)
Sample Volume 300 - 600 µl
Protein Loaded 405 µg
It is also suggested that the rehydration buffer should be equal or more
than 300 µl.
Bradford results belonging to Bortezomib and control group are represented in
Table 3.4. Bradford results for protein samples
Sample Name Protein Concentration
Bortezomib (17 nM) 10.20 µg / µl
Control 10.36 µg / µl
By taking notice of the recommended value in Table 3.3 and the concentration
of two protein samples (Bortezomib and control group) in Table 3.4, protein samples
were reconstituted with rehydration buffer. The reorganized sample values can be seen
in Table 3.5.
Sample
Name
Bortezomib
(17 nM)
Table 3.5. Reconstituted sample values
Sample Volume Protein
40 µl + 360 µl Rehydration Buffer = 400 µl
Loaded
408 µg
Control 40 µl + 360 µl Rehydration Buffer = 400 µl 414.4 µg
According to reorganized sample values, proteins were pipetted along the whole
length of the rehydration tray channel except for about 1 cm at each end. For this
process it was taken care not to introduce any bubbles that might interfere with the even
distribution of protein samples in the strip. Channels of the rehydration tray was
59