changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
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Table 3.4.<br />
Table 3.3. Required Prote<strong>in</strong> Sample with Rehydration Buffer<br />
Strip Length 17 cm (pH 3 - 10)<br />
Sample Volume 300 - 600 µl<br />
Prote<strong>in</strong> Loaded 405 µg<br />
It is also suggested that the rehydration buffer should be equal or more<br />
than 300 µl.<br />
Bradford results belong<strong>in</strong>g to Bortezomib and control group are represented <strong>in</strong><br />
Table 3.4. Bradford results for prote<strong>in</strong> samples<br />
Sample Name Prote<strong>in</strong> Concentration<br />
Bortezomib (17 nM) 10.20 µg / µl<br />
Control 10.36 µg / µl<br />
By tak<strong>in</strong>g notice of the recommended value <strong>in</strong> Table 3.3 and the concentration<br />
of two prote<strong>in</strong> samples (Bortezomib and control group) <strong>in</strong> Table 3.4, prote<strong>in</strong> samples<br />
were reconstituted with rehydration buffer. The reorganized sample values can be seen<br />
<strong>in</strong> Table 3.5.<br />
Sample<br />
Name<br />
Bortezomib<br />
(17 nM)<br />
Table 3.5. Reconstituted sample values<br />
Sample Volume Prote<strong>in</strong><br />
40 µl + 360 µl Rehydration Buffer = 400 µl<br />
Loaded<br />
408 µg<br />
Control 40 µl + 360 µl Rehydration Buffer = 400 µl 414.4 µg<br />
Accord<strong>in</strong>g to reorganized sample values, prote<strong>in</strong>s were pipetted along the whole<br />
length of the rehydration tray channel except for about 1 cm at each end. For this<br />
process it was taken care not to <strong>in</strong>troduce any bubbles that might <strong>in</strong>terfere with the even<br />
distribution of prote<strong>in</strong> samples <strong>in</strong> the strip. Channels of the rehydration tray was<br />
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