changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
You also want an ePaper? Increase the reach of your titles
YUMPU automatically turns print PDFs into web optimized ePapers that Google loves.
ABSTRACT<br />
CHANGES IN PROTEIN PROFILES IN BORTEZOMIB<br />
APPLIED MULTIPLE MYELOMA CELLS<br />
Multiple Myeloma is a malignant B-cell neoplasm that is characterized by the<br />
accumulation of malignant plasma cells <strong>in</strong> the bone marrow. Over the recent years,<br />
several novel agents have been <strong>in</strong>troduced <strong>in</strong> the treatment of this disease. Bortezomib<br />
is the first of a new class of agents known as proteasome <strong>in</strong>hibitors.<br />
The ma<strong>in</strong> objective of the project was basically to both determ<strong>in</strong>e the cytotoxic<br />
and apoptotic effects of Bortezomib on Multiple Myeloma U-266 cells and compare and<br />
explore the differences between Bortezomib <strong>applied</strong> Multiple Myeloma cells and<br />
control group Multiple Myeloma cells, by proteomics studies. In order to achieve our<br />
aims <strong>in</strong> the project, variety of multidiscipl<strong>in</strong>ary subjects were come together. Cancer<br />
research techniques, biochemical studies at prote<strong>in</strong> level and proteomics were comb<strong>in</strong>ed<br />
<strong>in</strong> our studies.<br />
In this study, our experimental results demonstrated that Bortezomib has<br />
antiproliferative and apoptotic effects on MM U-266 cells. On the other hand, the<br />
responsible prote<strong>in</strong>s for the effect mechanism of anti-cancer agent on cells were<br />
determ<strong>in</strong>ed by MALDI-TOF-TOF Mass Spectrometry for the first time. Accord<strong>in</strong>g to<br />
the mass spectrometric analysis, 37 prote<strong>in</strong> spots were differentially expressed. Among<br />
them, five prote<strong>in</strong>s were newly formed, ten prote<strong>in</strong>s lost, twelve prote<strong>in</strong>s were up-<br />
regulated and ten prote<strong>in</strong>s were down-regulated as compared to control group (untreated<br />
cells).These differential expressed prote<strong>in</strong>s <strong>in</strong> response to Bortezomib have different<br />
important functions rang<strong>in</strong>g from cell signal<strong>in</strong>g transduction, cell cycle regulation,<br />
apoptosis to immunity and defense mechanism.<br />
In conclusion, it was identified which prote<strong>in</strong>s have a central role beh<strong>in</strong>d the<br />
effect of Bortezomib on MM U-266 cells. The identified prote<strong>in</strong>s may let to be possible<br />
to treat other cancer types by same anticancer agent. The data obta<strong>in</strong>ed by this study<br />
may also be helpful for medical schools and drug designers and may also provide new<br />
treatments.<br />
iv