changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
changes in protein profiles in bortezomib applied multiple myeloma ...
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B.4.3. Standard Curve for BSA<br />
Table B.3. Absorbance Values for BSA Standards<br />
Concentrations<br />
(µg/µl)<br />
Absorbances<br />
(595 nm)<br />
1 0,1775<br />
2 0,182<br />
4 0,191<br />
8 0,209<br />
20 0,263<br />
40 0,353<br />
Figure B.3. Standard Curve for BSA<br />
Figure B.1. Standard Curve for BSA<br />
B.4.4. Coomassie Brilliant Blue G-250 (CBB G-250) Dye<br />
100 mg Coomassie Brilliant Blue G-250 (CBB G-250)<br />
50 ml Absolute Ethanol<br />
100 ml 85% Phosphoric Acid<br />
The above-mentioned mass of CBB G-250 was weighed and dissolved <strong>in</strong> 50 ml<br />
of absolute ethanol. Then, 100 ml of 85% Phosphoric Acid was added to this solution.<br />
After the overall solution was filtered through filter paper, the filtered solution (~15-20<br />
ml) was diluted to 1 liter with ultrapure water and stored at 4 o C.<br />
y = 0.1730 + 0.0045x<br />
R 2<br />
= 0.9966<br />
106