pdf - A test of psbK-psbI and atpF-atpH as potential plant DNA - ArBOL

Nature Precedings : hdl:10101/npre.2008.1896.1 : Posted 16 May 2008
In a multi loci approach for DNA barcoding purposes, the highest mean of inter-specific
variability was achieved by matK combined with trnH-psbA and atpF-atpH whereas the
highest mean of intra-specific distances were given by combining matK with trnH-psbA
(Table 3). Wilcoxon statistical rank tests showed the combination matK + trnH-psbA
having the highest inter-specific pair-distances (Table 4). They revealed also that all the
combinations including trnH-psbA had a higher intra-specific variability than
combinations without it (Table 5).
Distribution of distances. Accuracy of each DNA barcode was assessed by looking at the
distribution of inter- and intraspecific K2P distances to infer the barcoding gap 23 .
Distributions were similar for each single potential barcode with two peaks of inter- and
intraspecific variability that could be distinguished (Figure 2).
Figure 2. Relative distributions of inter-specific divergence between con-generic species (yellow)
and intra-specific K2P distances (red) for four single loci: matK, trnH-psbA, psbK-psbI and atpFatpH.
Barcoding gaps were assessed with Median tests and Wilcoxon Two-Sample tests, and all
were highly significant (p