Citrullus lanatus (Thunb.) Matsum. & Nakai - Cucurbit Breeding ...

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Yellow Belly.........................................................................................................................................................40 Intermittent Stripes .............................................................................................................................................41 Canary Yellow Flesh ..........................................................................................................................................41 Salmon Yellow and Red Flesh............................................................................................................................42 Spotted Rind ........................................................................................................................................................42 Conclusions .............................................................................................................................................................44 Literature Cited........................................................................................................................................................45 FOUNDATIONS OF YIELD IMPROVEMENT IN WATERMELON .................................................................61 Abstract....................................................................................................................................................................62 Introduction .............................................................................................................................................................62 Materials and Methods............................................................................................................................................64 Results and Discussion ...........................................................................................................................................66 Literature Cited........................................................................................................................................................69 HERITABILITY AND GENETIC VARIANCE ESTIMATES FOR FRUIT WEIGHT IN WATERMELON ..79 Abstract....................................................................................................................................................................80 Introduction .............................................................................................................................................................80 Materials and Methods............................................................................................................................................82 Germplasm and Crosses.....................................................................................................................................82 Cultural Practices...............................................................................................................................................83 Experimental design and Data Analysis ...........................................................................................................83 Results and Discussion ...........................................................................................................................................85 Literature Cited........................................................................................................................................................88 HERITABILITY AND GENETIC VARIANCE ESTIMATES FOR RESISTANCE TO GUMMY STEM BLIGHT IN WATERMELON .................................................................................................................................100 Abstract..................................................................................................................................................................101 Introduction ...........................................................................................................................................................101 Materials and Methods..........................................................................................................................................103 vii
Germplasm and Crosses...................................................................................................................................103 Inoculum Preparation ......................................................................................................................................103 Cultural Practices and Inoculation Methods..................................................................................................104 Disease Assessment ..........................................................................................................................................105 Experimental Design and Data Analysis ........................................................................................................106 Results and Discussion .........................................................................................................................................108 Conclusions ...........................................................................................................................................................110 Literature Cited......................................................................................................................................................111 PRELIMINARY STUDY OF MOLECULAR MARKERS LINKED TO RESISTANCE TO GUMMY STEM BLIGHT IN WATERMELON .................................................................................................................................128 Abstract..................................................................................................................................................................129 Introduction ...........................................................................................................................................................129 Materials and Methods..........................................................................................................................................133 Germplasm and Crosses...................................................................................................................................133 Inoculum Preparation ......................................................................................................................................133 Cultural Practices.............................................................................................................................................134 Inoculation Methods.........................................................................................................................................135 Disease Assessment ..........................................................................................................................................135 Leaf Collection and DNA Extraction ..............................................................................................................135 Design and Sources of PCR Primers...............................................................................................................136 Polymerase Chain Reaction Amplifications ...................................................................................................136 Gel Electrophoresis ..........................................................................................................................................137 Experimental design and Data Analysis .........................................................................................................138 Results and Discussion .........................................................................................................................................139 Future Research.....................................................................................................................................................142 Literature Cited......................................................................................................................................................143 GENERAL CONCLUSIONS ...................................................................................................................................165 viii
Page 1 and 2: Abstract GUSMINI, GABRIELE. Inherit
Page 3 and 4: Biography Gabriele Gusmini was born
Page 5 and 6: Currently, Gabriele is planning his
Page 7: Table of Contents LIST OF TABLES...
Page 11 and 12: GENERAL INTRODUCTION LIST OF TABLES
Page 13 and 14: GENERAL INTRODUCTION LIST OF FIGURE
Page 15 and 16: HERITABILITY AND GENETIC VARIANCE E
Page 17 and 18: Brief History of Watermelon Breedin
Page 19 and 20: absence of trichomes on the leaves
Page 21 and 22: The genetics of the flesh color in
Page 23 and 24: The primary nematode species attack
Page 25 and 26: 1975), but no gene has been found f
Page 27 and 28: Henderson, W.R., G.H. Scott, and T.
Page 29 and 30: Shimotsuma, M. 1963. Cytogenetical
Page 31 and 32: Table 1. Watermelon cultivars with
Page 33 and 34: Figure 2. Cultivar Allsweet, releas
Page 35 and 36: Figure 4. Watermelon seeds size: 1
Page 37 and 38: Introduction The inheritance of rin
Page 39 and 40: stripes. In his study, Shimotsuma c
Page 41 and 42: Diamond'), GG mm = mottled dark gre
Page 43 and 44: Shimotsuma, M. 1963. Cytogenetical
Page 45 and 46: Figure 2. Cultivars Japan 6 and Chi
Page 47 and 48: Figure 4. Cultivars Crimson Sweet (
Page 49 and 50: Abstract The watermelon [Citrullus
Page 51 and 52: The rind (skin) colors and patterns
Page 53 and 54: two hybrids are the original canary
Page 55 and 56: Names and symbols for new genes pro
Page 57 and 58: Salmon Yellow and Red Flesh During
Page 59 and 60:
During our experiments we did not f
Page 61 and 62:
Poole, C.F. and P.C. Grimball. 1945
Page 63 and 64:
Table 2. Single locus goodness-of-f
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Table 6. Continued. z Generation To
Page 71 and 72:
Figure 1. Intermittent stripes in '
Page 73 and 74:
Figure 3. Moons and stars induced o
Page 75 and 76:
Figure 5. Examples of unexpected fl
Page 77 and 78:
Abstract High yield is a major goal
Page 79 and 80:
States appears to be narrow, with m
Page 81 and 82:
y using only two replications from
Page 83 and 84:
value=0.0001), indicating that cult
Page 85 and 86:
Levi, A., C.E. Thomas, A.P. Keinath
Page 87 and 88:
Table 1. Analysis of variance (degr
Page 89 and 90:
Table 2. Continued. Yield z Fruit s
Page 91 and 92:
Table 2. Continued. Yield z Fruit s
Page 93 and 94:
w L/D = length/diameter ratio measu
Page 95 and 96:
Abstract The cultivated watermelon
Page 97 and 98:
dominance, and epistatic effects we
Page 99 and 100:
thumped (Maynard, 2001). Weights we
Page 101 and 102:
The giant-fruited parents had a lar
Page 103 and 104:
Literature Cited Brar, J.S. and K.S
Page 105 and 106:
Table 1. Phenotypic variances by ge
Page 107 and 108:
Table 2. Variance and heritability
Page 109 and 110:
t h 2 n = narrow-sense heritability
Page 111 and 112:
Table 3. Continued. Effective Facto
Page 113 and 114:
Figure 1. Each of the three cultiva
Page 115 and 116:
CHAPTER FIVE HERITABILITY AND GENET
Page 117 and 118:
fungus that is seed-borne (Lee et a
Page 119 and 120:
the technique described herein. Pyc
Page 121 and 122:
9 = plant dead. Plants with a disea
Page 123 and 124:
The possible gain from selection pe
Page 125 and 126:
Based on our data, Norton may not h
Page 127 and 128:
Gusmini, G., T.C. Wehner, and G.J.
Page 129 and 130:
Sherbakoff, C.C. 1917. Some importa
Page 131 and 132:
Page 133 and 134:
Table 1. Continued. z Generation To
Page 135 and 136:
w Expected was the hypothesized seg
Page 137 and 138:
on leaves only; 5 = some leaves dea
Page 139 and 140:
on leaves only; 5 = some leaves dea
Page 141 and 142:
z Data are ratings from four famili
Page 143 and 144:
CHAPTER SIX PRELIMINARY STUDY OF MO
Page 145 and 146:
fungus that is seed-borne (Lee et a
Page 147 and 148:
approach was successful in assembli
Page 149 and 150:
subculture on artificial medium bas
Page 151 and 152:
We extracted DNA using an extractio
Page 153 and 154:
products using the Genescan ® -500
Page 155 and 156:
(DNA/Polysaccharides) and 260/280 (
Page 157 and 158:
Future Research Based on our prelim
Page 159 and 160:
Gusmini, G., T.L. Ellington, and T.
Page 161 and 162:
Perin, C., L.S. Hagen, V. De Conto,
Page 163 and 164:
Table 2. Analysis of variance for t
Page 165 and 166:
029 v 1 . 3 3 . . 3 4 3 3 . . . + B
Page 167 and 168:
Table 3. Continued. 071 v 1 3 . 3 4
Page 169 and 170:
Table 3. Continued. 113 2 4 5 5 4 3
Page 171 and 172:
Table 3. Continued. 155 2 4 5 4 . 4
Page 173 and 174:
Page 175 and 176:
Page 177 and 178:
Figure 1. Agarose gel electrophores
Page 179 and 180:
1 5 10 15 20 25 30 35 + + + + + + +
Page 181 and 182:
The genetics of rind color (or patt
Page 183 and 184:
were not successful, even though se
Page 185 and 186:
Breeding Methods Traits of interest
Page 187 and 188:
alternative to biotechnology and te
Page 189 and 190:
The solution to these problems is n
Page 191 and 192:
Cucurbit Gene List Committee. 1979.
Page 193 and 194:
Henderson, W.R., G.H. Scott, and T.
Page 195 and 196:
Matthews, P. (ed.). 1993. The guinn
Page 197 and 198:
Provvidenti, R. 1991. Inheritance o
Page 199 and 200:
Souza, F.F.d., M.A.d. Queiroz, and
Page 201:
Wright, S. 1968. The genetics of qu
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