Citrullus lanatus (Thunb.) Matsum. & Nakai - Cucurbit Breeding ...

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The goal of our QTL mapping experiment would be to identify at least two markers flanking each QTL that explains large portions of the variation associated with resistance. Alternatively, should the single gene db be regulated by minor QTLs, a single molecular marker linked to db would be a useful tool to plant breeders for MAS. Ideally, the identification of a marker tightly linked to db and other markers detecting the presence of the minor QTLs would provide enough information to predict the phenotype of the progenies of each cross. Nevertheless, it could be difficult to achieve the correct mapping of these minor QTLs, due to the possibility that each of them explains too little of the total variation. Based on the results of this investigation, plant breeders could integrate MAS for the db gene and field and greenhouse testing to select the progenies with the highest expression of resistance under natural and artificial epidemics of gummy stem blight. Thus, minor QTLs could be selected directly by observation of the level of resistance in phenotypic testing. Field testing would be necessary for yield and quality traits, so that multiple years of trialing for disease resistance would be possible, prior to release of the best germplasm. Literature Cited Bruton, B.D. 1998. Soilborne diseases in Cucurbitaceae: pathogen virulence and host resistance, p. 143-166. In: J.D. McCreight (ed.). Cucurbitaceae 98. ASHS Press. Choi, H.K., D. Kim, T. Uhm, E. Limpens, H. Lim, J.H. Mun, P. Kalo, R.V. Penmetsa, A. Seres, O. Kulikova, B.A. Roe, T. Bisseling, G.B. Kiss, and D.R. Cook. 2004. A sequence-based genetic map of Medicago truncatula and comparison of marker collinearity with M. sativa. Genetics. 166: 1463-1502. de Vienne, D. (ed.). 2002. Molecular markers in plant genetics and biotechnology. Institute National de la Recherche Agronomique, Versailles, France. Dhingra, O.D. and J.B. Sinclair. 1995. Basic plant pathology methods. CRC press, Boca Raton, FL. Goodman, M.M. 2004. Plant breeding requirements for applied molecular biology. Crop Science. 44: 1913- 1919. Guerra-Sanz, J.M. 2002. Citrullus simple sequence repeats markers from sequence databases. Molecular Ecology Notes. 2: 223-225. 143
Gusmini, G., T.L. Ellington, and T.C. Wehner. 2003. Mass production of gummy stem blight spores for resistance screening. Cucurbit Genetics Cooperative Report. 26: 26-30. Gusmini, G., T. Joobeur, A. Levi, R.A. Dean, and T.C. Wehner. 2005a. Protocol for DNA extraction from watermelon leaves for SSR marker studies. Cucurbit Genetics Cooperative Report. In press. Gusmini, G., R. Song, and T.C. Wehner. 2005b. New sources of resistance to gummy stem blight in watermelon. Crop Science. in press. Gusmini, G. and T.C. Wehner. 2002. Correlation of ratings for resistance to gummy stem blight in watermelon, p. 199-205. In: J.D. McCreight (ed.). Cucurbitaceae 02. ASHS Press, Naples, FL. Gusmini, G., T.C. Wehner, and G.J. Holmes. 2002. Disease assessment scales for seedling screening and detached leaf assay for gummy stem blight in watermelon. Cucurbit Genetics Cooperative Report. 25: 36-40. Hashizume, T., I. Shimamoto, and M. Hirai. 2003. Construction of a linkage map and QTL analysis of horticultural traits for watermelon [Citrullus lanatus (THUNB.) MATSUM & NAKAI] using RAPD, RFLP and ISSR markers. Theoretical and Applied Genetics. 106: 779-785. Hawkins, L.K., F. Dane, T.L. Kubisiak, B.B. Rhodes, and R.L. Jarret. 2001. Linkage mapping in a watermelon population segregating for fusarium wilt resistance. Journal of the American Society for Horticultural Science. 126: 344-350. InforMax, I. 2002. GenoMax 3.4 [Online]. Available by InforMax, Inc. http://uncgene.unc.edu/genomax3/cgibin/UNCCH/genomax.cgi (verified 2004). Jarret, R.L., L.C. Merrick, T. Holms, J. Evans, and M.K. Aradhya. 1997. Simple sequence repeats in watermelon (Citrullus lanatus (Thunb.) Matsum. & Nakai). Genome. 40: 433-441. Katzir, N., Y. Danin Poleg, G. Tzuri, Z. Karchi, U. Lavi, and P.B. Cregan. 1996. Length polymorphism and homologies of microsatellites in several Cucurbitaceae species. Theoretical and Applied Genetics. 93: 1282-1290. Keinath, A.P. 1995. Fungicide timing for optimum management of gummy stem blight epidemics on watermelon. Plant Disease. 79: 354-358. Keinath, A.P. 1996. Soil amendment with cabbage residue and crop rotation to reduce gummy stem blight and increase growth and yield of watermelon. Plant Disease. 80: 564-570. 144
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Abstract GUSMINI, GABRIELE. Inherit
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Biography Gabriele Gusmini was born
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Currently, Gabriele is planning his
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Table of Contents LIST OF TABLES...
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Germplasm and Crosses..............
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GENERAL INTRODUCTION LIST OF TABLES
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GENERAL INTRODUCTION LIST OF FIGURE
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HERITABILITY AND GENETIC VARIANCE E
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Brief History of Watermelon Breedin
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absence of trichomes on the leaves
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The genetics of the flesh color in
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The primary nematode species attack
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1975), but no gene has been found f
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Henderson, W.R., G.H. Scott, and T.
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Shimotsuma, M. 1963. Cytogenetical
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Table 1. Watermelon cultivars with
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Figure 2. Cultivar Allsweet, releas
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Figure 4. Watermelon seeds size: 1
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Introduction The inheritance of rin
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stripes. In his study, Shimotsuma c
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Diamond'), GG mm = mottled dark gre
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Shimotsuma, M. 1963. Cytogenetical
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Figure 2. Cultivars Japan 6 and Chi
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Figure 4. Cultivars Crimson Sweet (
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Abstract The watermelon [Citrullus
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The rind (skin) colors and patterns
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two hybrids are the original canary
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Names and symbols for new genes pro
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Salmon Yellow and Red Flesh During
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During our experiments we did not f
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Poole, C.F. and P.C. Grimball. 1945
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Table 2. Single locus goodness-of-f
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Table 6. Continued. z Generation To
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Figure 1. Intermittent stripes in '
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Figure 3. Moons and stars induced o
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Figure 5. Examples of unexpected fl
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Abstract High yield is a major goal
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States appears to be narrow, with m
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y using only two replications from
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value=0.0001), indicating that cult
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Levi, A., C.E. Thomas, A.P. Keinath
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Table 1. Analysis of variance (degr
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Table 2. Continued. Yield z Fruit s
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Table 2. Continued. Yield z Fruit s
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w L/D = length/diameter ratio measu
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Abstract The cultivated watermelon
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dominance, and epistatic effects we
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thumped (Maynard, 2001). Weights we
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The giant-fruited parents had a lar
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Literature Cited Brar, J.S. and K.S
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Table 1. Phenotypic variances by ge
Page 107 and 108: Table 2. Variance and heritability
Page 109 and 110: t h 2 n = narrow-sense heritability
Page 111 and 112: Table 3. Continued. Effective Facto
Page 113 and 114: Figure 1. Each of the three cultiva
Page 115 and 116: CHAPTER FIVE HERITABILITY AND GENET
Page 117 and 118: fungus that is seed-borne (Lee et a
Page 119 and 120: the technique described herein. Pyc
Page 121 and 122: 9 = plant dead. Plants with a disea
Page 123 and 124: The possible gain from selection pe
Page 125 and 126: Based on our data, Norton may not h
Page 127 and 128: Gusmini, G., T.C. Wehner, and G.J.
Page 129 and 130: Sherbakoff, C.C. 1917. Some importa
Page 131 and 132: Table 1. Single locus goodness-of-f
Page 133 and 134: Table 1. Continued. z Generation To
Page 135 and 136: w Expected was the hypothesized seg
Page 137 and 138: on leaves only; 5 = some leaves dea
Page 139 and 140: on leaves only; 5 = some leaves dea
Page 141 and 142: z Data are ratings from four famili
Page 143 and 144: CHAPTER SIX PRELIMINARY STUDY OF MO
Page 145 and 146: fungus that is seed-borne (Lee et a
Page 147 and 148: approach was successful in assembli
Page 149 and 150: subculture on artificial medium bas
Page 151 and 152: We extracted DNA using an extractio
Page 153 and 154: products using the Genescan ® -500
Page 155 and 156: (DNA/Polysaccharides) and 260/280 (
Page 157: Future Research Based on our prelim
Page 161 and 162: Perin, C., L.S. Hagen, V. De Conto,
Page 163 and 164: Table 2. Analysis of variance for t
Page 165 and 166: 029 v 1 . 3 3 . . 3 4 3 3 . . . + B
Page 167 and 168: Table 3. Continued. 071 v 1 3 . 3 4
Page 169 and 170: Table 3. Continued. 113 2 4 5 5 4 3
Page 171 and 172: Table 3. Continued. 155 2 4 5 4 . 4
Page 177 and 178: Figure 1. Agarose gel electrophores
Page 179 and 180: 1 5 10 15 20 25 30 35 + + + + + + +
Page 181 and 182: The genetics of rind color (or patt
Page 183 and 184: were not successful, even though se
Page 185 and 186: Breeding Methods Traits of interest
Page 187 and 188: alternative to biotechnology and te
Page 189 and 190: The solution to these problems is n
Page 191 and 192: Cucurbit Gene List Committee. 1979.
Page 193 and 194: Henderson, W.R., G.H. Scott, and T.
Page 195 and 196: Matthews, P. (ed.). 1993. The guinn
Page 197 and 198: Provvidenti, R. 1991. Inheritance o
Page 199 and 200: Souza, F.F.d., M.A.d. Queiroz, and
Page 201: Wright, S. 1968. The genetics of qu
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