standardization of records keeping in indian zoos - Central Zoo ...

standardization of records keeping in indian zoos - Central Zoo ... standardization of records keeping in indian zoos - Central Zoo ...

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as the neck or under the wing can be selected instead. The area of the PIT tag implantation should be properly recorded and mentioned in the animal history sheet. As the PIT tags cannot provide instant recognition of the bird it should be used in combination with other marking system. Discussion Uses of dyes and ultraviolet markers, nasal discs and saddles, web tags etc are other methods for marking birds. These methods are unsuitable for use in zoos as dyes and ultraviolet markers only provide temporary identification to the birds and as for nasal discs and saddles various undesirable results have been reported, including high rates of marker loss, often with injury to the nares (Sherwood 1966), higher mortality rates attributed to entanglement with submerged vegetation (Sugden and Poston 1968) and reduced success in obtaining mates (Koob 1981).Visual identification of a web tagged waterfowl is impossible. A PIT tag in combination with other markers suitable for the specific species by far seems to be the best method for marking birds/ pheasants for identification. 6. MARKING OF AMPHIBIANS FOR IDENTIFICATION NEW TECHNIQUES 6.1 VISIBLE IMPLANT FLOURESCENT ELASTOMER (VIE) Amphibians are difficult to mark or individually identify. Their small size, sensitive and slippery skin, subtlety of their markings and the ability of the caudates and anurans to regenerate all contribute to these difficulties. A. VIE tagging system is bio- compatible and consists of two elastomer materials (color elastomer and curing agent) which are mixed at a 1:10 ratio. After mixing, the liquid elastomer material can be placed into a 0.3cc syringes and kept in a freezer to slow hardening, which occurs within 24 hours at room temperature. The 0.3cc syringes are used to inject the elastomer pigment into the animals. B. A total of six fluorescent colors (red, green, orange yellow, blue and pink) that fluoresce with the VI Light and four color that do not fluoresce (white, purple, brown and black) are available. Detection of fluorescent tags is greatly enhanced by using VI Light. Furthermore, colors green and yellow are difficult to distinguish in UV light thus either green or yellow should be used for each plot. In order to mark animals uniquely, fluorescent colors have to be combined with different tag locations (behind the front legs or in front of the hind legs or, if possible, in the legs itself). An injection into the legs shows up extremely well. Before and after injection, the syringe should be cleaned with an alcohol wipe to reduce the likelihood of infections. After marking tags should be checked for visibility using a hand held UV light and remarked if necessary. No anesthesia is needed. 58

Picture 22: Marking position in a salamander Picture 23:This frog tagged as a tadpole retained it‟s VIE tags through metamorphosis. Photo courtesy of S. Hopkins Picture 24: Photo courtesy K. Kendall, Alberta Fish and Wildlife Division Some amphibians lack septa between the skin and underlying tissue. VIE tags injected into these animalscan therefore migrate from the original tagginglocation, making it impossible to use those tagginglocations to create individual codes. In such cases, the use of Visible Implant Alpha tags is recommended. Representatives of all the following families have been successfully tagged with VIE; Amphibians Ambystomatidae Ascaphidae Caeciliidae – caecelians Hylidae – tree frogs 59

Picture 22: Mark<strong>in</strong>g position <strong>in</strong> a salamander<br />

Picture 23:This frog tagged as a tadpole reta<strong>in</strong>ed it‟s VIE tags through metamorphosis. Photo courtesy<br />

<strong>of</strong> S. Hopk<strong>in</strong>s<br />

Picture 24: Photo courtesy K. Kendall, Alberta Fish and Wildlife Division<br />

Some amphibians lack septa between the sk<strong>in</strong> and underly<strong>in</strong>g tissue. VIE tags <strong>in</strong>jected <strong>in</strong>to<br />

these animalscan therefore migrate from the orig<strong>in</strong>al tagg<strong>in</strong>glocation, mak<strong>in</strong>g it impossible to<br />

use those tagg<strong>in</strong>glocations to create <strong>in</strong>dividual codes. In such cases, the use <strong>of</strong> Visible Implant<br />

Alpha tags is recommended.<br />

Representatives <strong>of</strong> all the follow<strong>in</strong>g families have been successfully tagged with VIE;<br />

Amphibians<br />

Ambystomatidae<br />

Ascaphidae<br />

Caeciliidae – caecelians<br />

Hylidae – tree frogs<br />

59

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