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Adapt<strong>at</strong>ion of <strong>Shigella</strong> to the gut environment<br />
<strong>Shigella</strong> is protected against wasteful secretion in gut<br />
anaerobic environment. Marteyn et al., N<strong>at</strong>ure, 2010<br />
TTSS<br />
MxiH<br />
IpaB<br />
IpaC<br />
- FNR-dependent inactiv<strong>at</strong>ion<br />
of Spa32/33 expression<br />
- No switch in substr<strong>at</strong>es (MxiH Ipas)<br />
- Elong<strong>at</strong>ion of needles<br />
- Secretion of Ipas blocked<br />
- Accumul<strong>at</strong>ion of Ipas<br />
Secretion signal ?<br />
« incompetence /<br />
priming »<br />
FNR<br />
Fe/S Fe/S<br />
X<br />
GFP-expressing E.coli<br />
Actin/LPS<br />
DAPI<br />
Intestinal lumen<br />
Epithelium<br />
- FNR does not bind to<br />
Spa32/33 regul<strong>at</strong>ors<br />
- Switch in substr<strong>at</strong>es<br />
(MxiH Ipas)<br />
- Secretion of Ipas upon<br />
signal<br />
« competence »<br />
ApoFNR<br />
spa32/33 spa32/33<br />
TTGATATCAA<br />
100-200 mm<br />
Secretion signal<br />
3-5 mmHg<br />
20-70 mmHg<br />
O 2
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