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other cucurbits. SNP frequency was found to vary between one in 5000 bp to ten in 300 bp. A search for linkages between SNPs and traits is underway using a RIL population derived from the F2 population. Acknowledgements: Contribution no. 108/2007 of the Institute of Plant Sciences, Agricultural Research Organization, Israel. Literature Cited: 1. Danin-Poleg, Y., N. Reis, S. Baudracco- Arnas, M. Pitrat, J.E. Staub, M. Oliver, P. Arus, C.M. deVicente, and N. Katzir. 2000. Simple Sequence Repeats in Cucumis mapping and map merging. Genome 43: 963-974. 2. Danin-Poleg, Y., Y. Tadmor, G. Tzuri, N. Reis, J. Hirschberg, and N. Katzir. 2002. Construction of a genetic map of melon with molecular markers, horticultural traits and ZYMV resistance. Euphytica 125: 373-384. 3. Gonzalo, M. J., M. Oliver, J. Garcia- Mas, A. Monfort, R. Dolcet-Sanjuan , N. Katzir, P. Arús, and A. J. Monforte. 2005. Simple-sequence repeat markers used in merging linkage maps of melon (Cucumis melo L.). Theor. Appl. Genet. 110: 802-811. 4. Gut, I. G. 2001. Automation in genotyping of single nucleotide polymorphisms. Hum. Mutat. 17: 475- 492. 5. Oeth, P., M. Beaulieu, C. Park, D. Kosman, G. del Mistro, D. van den Boom, and C. Jurinke. 2005. iPLEX TM Assay: Increased plexing efficiency and flexibility for MassARRAY® System through single base primer extension with mass-modified terminators. Sequenom application note (http://www.sequenom.com/Seq_genoty ping.html). 6. Périn, C., L. Hagen, V. De Conto, N. Katzir, Y. Danin-Poleg, V. Portnoy, S. Baudracco-Arnas, J. Chadoeuf, C. Dogimont, and M. Pitrat. 2002. A reference map of Cucumis melo based on two recombinant inbred line populations. Theor. Appl. Genet. 104: 1017-1034. 7. Silberstein, L.,I. Kovalski, Y. Brotman, C. Perin, C. Dogimont, M. Pitrat, J. Klingler, G. Thompson, V. Portnoy, N. Katzir, and R. Perl-Treves, 2003. Linkage map of Cucumis melo including phenotypic traits and sequencecharacterized genes. Genome 46: 761- 773. 20 Cucurbit Genetics Cooperative Report 28-29: 19-21 (2005-2006)
Table 1. Genes associated with carbohydrate, carotenoid and organic acid metabolism. SNPs were developed for all the listed genes. Stars mark the genes located on the melon map. Alkaline alpha galactosidase I * Alkaline alpha galactosidase 2 * Acid alpha galactosidase 1 * Putative galactose kinase Galactose-1-phosphate uridyltransferase * UDP-glucose 4epimerase 1 UDP-glucose 4epimerase 2 UDP-glucose 4epimerase 3 UDP-glucose pyrophosphorylase *Phosphoglucomutase (cytosol) * Sucrose-phosphate synthase 1 Putative sucrose transporter Sugar transporter superfamily Carbohydrate genes Hexose transporter 1 * Hexose transporter 6 * Acid invertase 1 * Acid invertase 2 *Alkaline/ neutral invertase 1 *Alkaline/ neutral invertase 2 * Sucrose synthase Sucrose synthase 1 * Sucrose cleavage protein-like Hexokinase 2 Fructokinase 3 Carotenoid genes * Geranylgeranyl Pyrophosphate reductase Geranylgeranyl pyrophosphate synthase * Phytoene synthase 1 * Phytoene synthase 2 * Phytoene desaturase * Zeta carotene desaturase * Lycopene betacyclase * Βeta-carotene hydroxilase * Carotenoid isomerase Phosphoenol pyruvate carboxykinase * Phosphoenol pyruvate carboxylase (a) * Phosphoenol pyruvate carboxylase (b) * Citrate synthase (glyoxysome) * Citrate synthase (mitochondria) * Aconitase * NAD Isocitrate dehydrogenase (β) * α keto glutarate dehydrogenase (E2) * Succinyl CoA synthetase (α) * Succinyl CoA synthetase (β) * Succinate dehydrogenase (α) * Succinate dehydrogenase (β) * Fumarase Organic acid genes NAD malate dehydrogenase (mitochondria) NAD malate dehydrogenase (glyoxysome) * NADP malic enzyme (chloroplast) * NAD malate dehydrogenase (chloroplast) * Malate synthase (glyoxysome) * NADP Isocitrate lyase ATP citrate lyase (b) NADP malic enzyme (cytosol) * NAD Isocitrate dehydrogenase * Citrate transporter (mitochondria) *Citrate transporter (glyoxysome) * Vacuolar pump V0-a subunit * Vacuolar pump V0-c' subunit * Vacuolar pump V0-c subunit * Vacuolar pump V0-d subunit * Vacuolar pump V0-e subunit * Vacuolar pump V1-a subunit * Vacuolar pump V1-b subunit Vacuolar pump V1-C subunit * Vacuolar pump V1-d subunit * Vacuolar pump V1-e subunit * Vacuolar pump V1-f subunit Vacuolar pump V1-G subunit Vacuolar H[+]translocating inorganic pyrophosphatase Cucurbit Genetics Cooperative Report 28-29: 19-21 (2005-2006) 21
Page 1 and 2: Cucurbit Genetics Cooperative 28-29
Page 3 and 4: NEWS & COMMENT v 30th Annual CGC Bu
Page 5 and 6: 30th Annual CGC Business Meeting (2
Page 7 and 8: and emerging problems facing the se
Page 9 and 10: • R.L. Hassell, J. Schultheis, W.
Page 13 and 14: Control of Cucumber Grey Mold by En
Page 15 and 16: Discussion: Microbial endophytes ar
Page 17 and 18: Table 2. The effects of endophytic
Page 19 and 20: Systemic Resistance against Sphaero
Page 21 and 22: Results: Systemic induced resistanc
Page 23 and 24: Table1. Effects of inducers on the
Page 25 and 26: on visual appearance, cavities appe
Page 27 and 28: Literature Cited: 1. Danin-Poleg, Y
Page 29 and 30: A Recessive Gene for Light Immature
Page 31: SNP Discovery and Mapping in Melon
Page 35 and 36: used to check the marker order. Map
Page 37 and 38: 0 130 0 40 8 1 OJ04.1000 OH03.500 O
Page 39 and 40: the >Deltex= x TGR1551 cross in the
Page 41 and 42: Number of F2 plants Number of F2 pl
Page 43 and 44: Mapping of QTL for Fruit Size and S
Page 45 and 46: Table 2. Simple linear regression (
Page 47 and 48: Grafted Watermelon Stand Survival A
Page 49 and 50: ace, color, national origin, religi
Page 51 and 52: Rootstock Effects on Plant Vigor an
Page 53 and 54: Diann Baze and Dr. Benny Bruton for
Page 55 and 56: Pilot Survey Results to Prioritize
Page 57 and 58: Table 1. Results of the research ne
Page 59 and 60: A New Male Sterile Mutant Identifie
Page 61 and 62: Spontaneous Mutant Showing Pale See
Page 63 and 64: Figure 1. Comparison of pale green
Page 65 and 66: Genetic resistance to insect pests
Page 67 and 68: dominance being the largest gene ef
Page 69 and 70: watermelon (Citrullus lanatus (Thun
Page 71 and 72: gourd and watermelon. Proceedings o
Page 73 and 74: Figure 2. Watermelon seed size: 1 =
Page 75 and 76: Literature Cited: 1. Molinar, R., a
Page 77 and 78: Figure 2. Cultivar Allsweet, releas
Page 79 and 80: hermaphrodita, Momordica charantia,
Page 81 and 82: local farmers. One has dark green f
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amplified in C. moschata and C. max
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Inheritance of Yellow Seedling Leth
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Another Relationship between Stem a
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Diversity within Cucurbita maxima a
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Precocious Yellow Rind Color in Cuc
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Contrary to what has been reported
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Figure 1. Range of mature fruit col
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Results: A total of 176 PI accessio
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Table 1. Sample size (n) and mean (
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Figure 3. Fruit and seed of Cucumis
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Genetic Variability in Ascorbic Aci
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Table 1.Total carotenoid and ascorb
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PBOG 54, PBOG 61, PBOG 76, PBOG 117
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additive gene action in the inherit
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Components / proportions Internodal
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Cucurbit Genetics Cooperative Repor
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Cucurbit Genetics Cooperative Repor
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Gene List 2005 for Cucumber Todd C.
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that a single recessive gene mp was
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Jones, 1971a; Soans et al., 1973).
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CsP440s/E1, CsP221/H3, CsC625/E1, C
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ecombination values. Youngner (1952
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Zijlstra (1987) also determined tha
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co - green corolla. Green petals th
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observed. Fl - Fruit length. Expres
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m-2 h andromonoecious-2. Bisexual f
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susceptibility. Pm-h from 'Wis. SMR
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white (WfWf YfYf ): wf from 'NPI '
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library DNA fragment Jayabaskaran,
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AB026821 Seedling RNA Encoding IAA
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Cochran, F. D. 1938. Breeding cucum
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cDNA sequence and very early expres
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esistance and powdery mildew lysozy
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Pyzenkov, V. I. and G. A. Kosareva.
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Shiomi, S., M. Yamamoto, T. Ono, K.
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for bitterfree foliage in cucumber.
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Acp-1 APS-11, Ap-1 1 Acid phosphata
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Fom-2 Fom1.2 Fusarium oxysporum mel
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ud stage (in Bulgaria 7). ms-5 - ma
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Pm-7 - Powdery mildew resistance-7.
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Y - Yellow epicarp. Dominant to whi
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Cm-AO4 AF233594 Ascorbate oxidase A
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Cm-XTH2 DQ914795 Xyloglucan endotra
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to downy mildew in Cucumis melo PI
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58. Jagger, I.C., T.W. Whitaker and
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99. Périn, C., L.S. Hagen, V. de C
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140. Zink, F.W. 1986. Inheritance o
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Aboul-Nasr, M. Hossam. Dept. Hortic
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Interests: myrothecium stem canker
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& 724966; Interests: cucumber, melo
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Kuginuki, Yasuhisa. National Instit
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Email: lmerrick@iastate.edu; Ph: 51
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Randhawa, Lakhwinder. Sakata Seed A
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Thompson, Gary A. Univ. Arkansas LR
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Alabama Dane, F Arkansas Morelock,
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Peru Holle, M Philippines Beronilla
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ARTICLE IV. Election and Appointmen
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