journal of forest science

Table 4. Regression analyses: influence of altitude on the quality
of the Swiss Mountain Pine seeds during the second year of
investigations (Kleè II)
y = 139.695 – 0.057 x 1 1 r = –0.508 r2 = 0.258*
y = 155.102 – 0.063 x 2 1 r = –0.314 r2 = 0.099
y = 0.110 + 1.389.10 4 –3 x1 r = 0.079 r2 = 0.006
y = 77.453 – 0.048 x 5 1 r = –0.553 r2 = 0.306*
y = 96.730 – 0.027 x 6 1 r = –0.105 r2 = 0.011
y = 3.437 – 1.250·10 7 –3 x1 r = –0.077 r2 = 0.006
Note: y – content of full seeds; y – anatomical analysis; y – bioche-
1 2 3
mical analysis; y – polyembryony; y – germinative energy; y – ger-
4 5 6
mination; y – anomaly of germination; r – correlation coefficient;
7
x – altitude; * – significant correlation between variables
1
germination tests were observed in a majority of the samples,
accounting for 8.3% (ig. 1c).
Statistical analyses (Table 6) document significant correlations
between altitude and vitality (biochemically
analysed seeds) and between polyembryony and germinative
energy. No statistically significant correlation was
determined between altitude and germination.
The fourth year of investigations
Table 7 shows the results of these analyses. Content of
full seeds amounted to 54–78.3%. Content of vital seeds
according to the anatomical analyses ranged from 25.8%
to 96% and according to the biochemical analyses from
24.3% to 94.8%. Polyembryony was evident in all samples
and its maximal value was 7%. Tests of germination
show that germinative energy amounted to 4.5–34.5%
Table 6. Regression analyses: influence of altitude on the quality
of the Swiss Mountain Pine seeds during the third year of
investigations (Kleè III)
y = 110.709 – 0.029 x 1 1 r = –0.306 r2 = 0.094
y = 138.622 – 0.052 x 2 1 r = –0.269 r2 = 0.073
y = 154.238 – 0.066 x 3 1 r = –0.352 r2 = 0.124*
y = –11.679 + 0.011 x 4 1 r = 0.521 r2 = 0.271*
y = 94.069 – 0.060 x 5 1 r = –0.502 r2 = 0.252*
y = 151.983 – 0.073 x 6 1 r = –0.292 r2 = 0.085
y = 1.690 + 1.112·10 7 –3 x1 r = 0.053 r2 = 0.003
Note: y – content of full seeds; y – anatomical analysis; y – bioche-
1 2 3
mical analysis; y – polyembryony; y – germinative energy; y – ger-
4 5 6
mination; y – anomaly of germination; r – correlation coefficient;
7
x – altitude; * – significant correlation between variables
1
and germination to 7.8–93.3%. Anomalies during the
germination tests were similar to those of previous tests
(maximal value – 4.8%).
Statistical analyses (Table 8) show significant correlations
between altitude and content of full seeds and other
factors, but with the exception of polyembryony.
All 4 years of investigations into the qualitative characteristics
provide these common results: incompletely
developed embryos, polyembryony, anomalies during
germination tests, significant correlation between altitude
and content of full seeds, no correlation with polyembryony,
no similar trend in other qualitative characteristics
during the four years of study.
These results are important for a more complex study
to be carried out in future.
Origin of samples: Kleè 1 – Luèní hora; Kleè 2 –
Støíbrná bystøina; Kleè 3 – Šarfova bouda; Kleè 4 – Úpské
Table 5. Results of qualitative analyses and germination tests of the Swiss Mountain Pine seeds during the third year of investigations
(Kleè III)
Altitude Sample Content Anatomical analyses (%) Biochemical analyses (%) Germination tests (%)
(m a.s.l.) of full vital nonvital vital nonvital polyem- germinative germination anomalies
seeds (%) bryony energy
1,530 Kleè 1 45.8 39.5 57.5 34.5 56.5 9.0 4.0 7.3 0.3
1,480 Kleè 2 70.3 92.5 7.5 82.3 15.2 2.5 25.3 84.3 4.3
1,460 Kleè 3 64.5 36.8 63.2 35.3 62.4 2.3 3.0 17.8 0.0
1,420 Kleè 4 80.0 85.8 14.2 79.5 18.2 2.3 5.0 65.0 8.3
1,400 Kleè 5 75.8 69.8 28.2 67.8 23.7 8.5 12.0 50.0 3.8
1,390 Kleè 6 52.8 88.0 10.0 84.0 11.2 4.8 21.0 78.3 4.5
1,370 Kleè 7 82.8 55.5 44.5 53.3 43.2 3.5 5.5 36.8 2.0
1,360 Kleè 8 83.5 84.5 15.5 83.3 13.9 2.8 20.0 38.3 7.5
1,340 Kleè 9 60.5 91.0 9.0 80.8 17.9 1.3 28.8 94.0 4.5
1,320 Kleè 10 83.5 73.5 26.5 74.3 19.7 6.0 8.0 66.3 3.8
1,310 Kleè 11 92.0 32.3 67.7 22.3 77.7 0.0 1.0 5.0 0.3
1,300 Kleè 12 80.8 87.0 13.0 84.3 11.7 4.0 11.3 59.8 1.8
1,270 Kleè 13 63.5 32.3 67.7 24.5 75.2 0.3 3.3 15.5 0.0
1,265 Kleè 14 83.8 35.3 64.7 28.5 69.5 2.0 0.8 13.5 0.3
1,250 Kleè 15 74.0 84.0 16.0 77.5 15.7 6.8 14.5 74.5 5.5
1,200 Kleè 16 59.0 93.3 6.7 87.8 9.2 3.0 18.8 80.0 1.0
1,150 Kleè 17 65.3 92.5 7.5 85.8 13.9 0.3 28.3 92.3 6.3
1,120 Kleè 18 72.0 94.0 6.0 98.5 1.5 0.0 50.5 97.5 4.3
1,100 Kleè 19 82.0 92.8 7.2 92.3 7.4 0.3 50.5 65.5 1.0
J. FOR. SCI., 47, 2001 (7): 294–300 297