journal of forest science

Table 1. Results of qualitative analyses and germination tests of the Swiss Mountain Pine seeds during the first year of investigations
(Kleè I)
Altitude Sample Content Anatomical analyses (%) Biochemical analyses (%) Germination tests (%)
(m a.s.l.) of full vital nonvital vital nonvital polyem- germinative germination anomalies
seeds (%) bryony energy
1,530 Kleè 1 43.0 35.0 65.0 28.3 64.9 6.8 1.5 6.5 0.8
1,480 Kleè 2 74.8 96.3 3.7 94.8 1.4 3.8 19.5 94.5 5.5
1,460 Kleè 3 60.3 33.3 63.7 25.0 74.2 0.8 1.8 16.8 0.5
1,420 Kleè 4 72.0 81.0 19.0 76.3 22.9 0.8 1.5 47.8 1.8
1,400 Kleè 5 72.3 66.3 33.7 61.8 31.9 6.3 15.8 58.5 4.5
1,390 Kleè 6 46.0 78.5 20.0 77.3 16.2 6.5 7.5 76.0 5.8
1,370 Kleè 7 71.0 48.8 51.2 47.8 51.7 0.5 1.8 42.0 0.5
1,360 Kleè 8 82.5 82.5 17.5 85.0 9.5 5.5 12.5 83.3 5.3
1,340 Kleè 9 57.3 99.0 1.0 96.5 2.0 1.5 38.0 98.3 1.5
1,320 Kleè 10 76.0 59.8 40.2 56.3 39.9 3.8 7.5 58.0 4.0
1,310 Kleè 11 93.3 16.3 83.7 12.5 87.2 0.3 0.5 3.5 0.0
1,300 Kleè 12 85.8 80.8 19.2 75.5 18.5 6.0 10.0 45.3 2.5
1,270 Kleè 13 68.5 24.0 76.0 23.5 74.2 2.3 3.0 10.5 0.5
1,265 Kleè 14 84.3 27.8 72.2 20.5 74.5 5.0 0.8 11.0 0.0
1,250 Kleè 15 73.3 79.0 21.0 76.5 16.0 7.5 10.5 77.8 2.8
1,200 Kleè 16 58.0 93.5 6.5 87.8 8.4 3.8 19.5 85.0 0.8
1,150 Kleè 17 58.0 96.0 4.0 97.3 2.7 0.0 27.8 98.3 8.0
1,120 Kleè 18 68.0 99.5 0.5 95.8 3.2 1.0 51.0 97.8 4.0
1,100 Kleè 19 80.0 96.5 3.5 92.5 7.0 0.5 47.8 89.8 2.0
Standard ÈSN 48 1211 Sbìr, jakost a zkoušky jakosti
plodù a semen lesních døevin [Collection, Quality and
Quality Tests of orest Tree ruits and Seeds]) were
carried out. Some anomalies during the process of germination
were observed. Data on the germinative
energy, germination, seed quality were statistically processed
by arithmetical means and regression analyses
in the different years of study (designated as Kleè I,
Kleè II, Kleè III and Kleè IV).
RESULTS
The results of anatomical analyses, biochemical analyses
and germination tests are given in Tables 1–8.
The first year of investigations
Data in Table 1 show that the content of full seeds was
between 43 and 93.3% in different samples, content of
vital seeds according to the anatomical analyses ranged
from 16.3% to 99.5% and according to the biochemical
analyses from 12.5% to 97.3%. The polyembryony was
evident in 0.3–7.5% of the samples (with the exception
of 1 sample). The results of germination tests show that
germinative energy was 0.5–51.0% and germination
amounted to 3.5–98.3%. Anomalies during the germination
tests were found in seeds of many samples and they
accounted for 8%. They were indicated by germination
of green parts of epicotyls (see ig. 1c).
ig. 1 illustrates the unusually developed and nongerminating
seeds and specifically germinating seed.
ig. 2 shows a completely developed embryo of the
Swiss Mountain Pine seed as seen under an electron microscope
REM-JSM-35-Jeol.
Statistical analyses (Table 2) document significant correlations
between altitude and content of full seeds and
significant correlations between altitude and the germinative
energy and germination. There was no statistically
significant correlation between altitude and polyembryony.
The second year of investigations
Results of these analyses are shown in Tables 3 and 4.
Content of full seeds ranged from 38.0% to 96.5%. Con-
Fig. 1. The incompletely developed embryos and the atypically
germinating Swiss Mountain Pine seed (a – polyembryony,
b – incompletely developed embryo, c – atypically germinating
seed)
J. FOR. SCI., 47, 2001 (7): 294–300 295