EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
Identification <strong>of</strong> wheat susceptibility factors to<br />
Fusarium graminearum<br />
C. Chetouhi, L. Bonhomme, F. Cambon, P. Lecomte, D. Biron, T. Langin<br />
E-mail: cherif.chetouhi@clermont.inra.fr<br />
Among the most damaging fungal pathogens <strong>of</strong> wheat, Fusarium graminearum,<br />
main causal agent <strong>of</strong> Fusarium head blight (FHB), reduces significantly yield<br />
worldwide, and affects grains quality by mycotoxins contamination. During the<br />
past years, numbers <strong>of</strong> FHB resistance QTL with moderate effects against this<br />
pathogen have been identified in very genetically diverse wheat collections.<br />
However the lack <strong>of</strong> diagnostic markers as well as detrimental linkage drag<br />
associated with some <strong>of</strong> these QTL (QTL governing the yield and grain quality)<br />
limit breeding for resistance. Understanding the molecular basis <strong>of</strong> FHB<br />
susceptibility can provide alternative tools to improve the control <strong>of</strong> this disease in<br />
fields. To decipher the molecular crosstalk involved during compatible interaction<br />
between F. graminearum and its host, and to gain information about susceptibility<br />
host factors, we analyzed a time course infection <strong>of</strong> the susceptible French wheat<br />
cultivar Recital by F. graminearum through transcriptomics and proteomics<br />
approaches. Microarray data showed that 1,453 genes exhibit differential<br />
accumulation between F. graminearum-infected and mock-inoculated plants<br />
whereas a total <strong>of</strong> 74 proteins displayed contrasting abundances on 2DE gels. All<br />
these host genes/proteins identified were classified into three functional groups:<br />
(i) plant defense, (ii) primary, secondary and energy metabolism and (iii)<br />
regulation and signaling. These results strongly suggest that F. graminearum<br />
manipulates its host during a compatible interaction. Its infection strategy relies on<br />
the suppression <strong>of</strong> basal plant defense and subtle changes in nutrient availability<br />
related-processes. A detailed picture <strong>of</strong> the potential host pathways involved<br />
during susceptibility will be described along with interesting targets for improved<br />
resistance.<br />
Keywords: Fusarium head blight (FHB), wheat, susceptibility<br />
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