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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,
VARIETY DEVELOPMENT
Identification of Frontana derived QTL linked to
Fusarium head blight, Fusarium damaged kernel and
deoxynivalenol content
Á. Szabó-Hevér, M. Varga, S. Lehoczki-Krsjak, C. Lantos, J. Pauk, L.
Purnhauser, Á. Mesterházy
Cereal Reseach Ltd., Alsó kikötő sor 9., H-6726, Szeged, Hungary
E-mail: agnes.szabo@gabonakutato.hu
There are numerous quantitative trait loci (QTL) studies in the research field of
wheat (Triticum aestivum L.) Fusarium resistance. However it is understandable
through the importance of this disease and the complexity of the resistance
against this pathogen. Beyond the Fusarium head blight (FHB) severity,
percentage of Fusarium damaged kernels (FDK) and deoxynivalenol (DON)
content are predominantly important resistance traits.
The physiological background of DON degradation in the moderately Fusarium
resistant Frontana variety was previously investigated by other research groups.
Additionally Frontana derived QTL influencing FHB and/or FDK were validated
using a Frontana/Remus DH population (Szabó-Hevér et al. 2012; Canadian
Journal of Plant Pathology, 34: 224–238).
In order to analyze QTL influencing DON content, a GK Mini Manó/Frontana DH
mapping population (n=168) was developed and investigated in field nurseries at
Szeged in 2008 and 2009. Spray inoculation combined with bagging method was
used to test FHB severity, FDK and DON content caused by independent
Fusarium isolates (F. culmorum or F. graminearum). Mapping was made with 643
polymorphic molecular markers (24 SSR and 619 DArT) constituting 28 linkage
groups.
The results show that Frontana derived QTL influencing DON accumulation are
on chromosomes: 1B, 2D, 3A, 3B, 4B, 5A, 5B, 6B, 7A, 7B and 7D. Among them
QTL identified on chromosomes 1B, 2D, 3B, 5A, 5B, 6B and 7B were the most
important influencing not only FHB and FDK but also DON content. Previous
results – gained through the mapping of Frontana/Remus population – support
the significant role of QTL on chromosomes 2D, 5A, 6B and 7B in the Fusarium
head blight resistance. The markers identified in these regions were stable across
different epidemic situations and linked to more resistance traits. Therefore their
use is beneficial for marker assisted selection.
Ackowledgements: This research was realized in the frames of TÁMOP 4.2.4.
A/1-11-1-2012-0001 „National Excellence Program – Elaborating and operating
an inland student and researcher personal support system” The project was
subsidized by the European Union and co-financed by the European Social Fund.
This material was supported by the EU MycoRed FP7 program.
Keywords: Triticum aestivum L., Fusarium head blight, deoxynivalenol, QTL
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