EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
Dose response study based in vitro selection of an
adsorbent capable to alleviate the negative in vivo
effects of zearalenone in female weaned piglets.
B. Vennekens, E. Schoeters, A. Teunckens, T. Vanderborght, N. Tallarico, M.
Baecke, S. Van Dyck
Kemin Europa NV, Toekomstlaan 42, 2200 Herentals, Belgium
E-mail: monique.baecke@kemin.com
In vitro analysis of mycotoxin adsorption is a powerful screening tool to evaluate
the potential of mycotoxin detoxifying adsorbents. The single concentration
method measures adsorption of a fixed mycotoxin concentration that is reacted
with a fixed concentration of adsorbent in an aqueous solution. Dose response
studies are a more elaborated test system where mycotoxin adsorption is
evaluated in function of varying adsorbent to mycotoxin ratios. From dose
response curves, the C50 value, the binder to mycotoxin ratio for which 50% of the
mycotoxin is bound, can be calculated. The in vitro zearalenone (ZEA) adsorption
of different adsorbents was evaluated with single concentration and dose
response studies. The more elaborated dose response studies offered more
differentiation potential than single concentration studies.
Based on the in vitro results, the adsorbent with the greatest potential was
evaluated in vivo for six weeks. Sixty female weaned piglets were randomly
assigned to five different treatments with three replicates within each treatment
and four piglets per replicate. The negative control treatment was fed a low ZEA
contaminated diet (0.065 ppm) while the four other treatments were fed a diet with
a ZEA contamination of about 1 ppm and an adsorbent dosage of 0, 1, 2 and 3
kg/T. In vivo ZEA adsorption, beta-estradiol serum concentration and vulva size
were recorded for all treatments. Histological analyses of follicular and acinar cells
were performed on uterus and ovary samples. Feeding female piglets with 1 ppm
ZEA exerted hyperestrogenism effects such as vulva swelling and depressed
serum beta-estradiol levels. Follicular and acinar cells were reduced after ZEA
exposure. The adsorbent was able to alleviate the ZEA induced effects to the
same level as in the negative control treatment. These observations were
supported by an increased in vivo ZEA adsorption of the adsorbent in a dose
dependent manner.
Keywords: zearalenone, adsorbent, in vitro, efficacy
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