EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
The type of interaction between type B
Trichothecenes on the intestine varies with the dose
I. Alassane-Kpembi 1,2,3 , M. Kolf-Clauw 1,2 , T. Gauthier 1,2 , R. Abrami 1,2 , F. A.
Abiola 3 , I. P. Oswald 1, 2* , O. Puel 1,2
1 INRA, UMR 1331 Toxalim, Research center in Food Toxicology, F-31027, Toulouse France;
2 Université de Toulouse, ENVT, INP, UMR 1331, Toxalim, F-31076, Toulouse, France; 3 Institut des
Sciences Biomédicales Appliquées, Cotonou, Bénin
E-mail: imourana.alassane@toulouse.inra.fr
Deoxynivalenol (DON) is the most prevalent trichothecene mycotoxin in crops in
Europe and North America. DON is often present with other type B trichothecenes
such as 3-acetyldeoxynivalenol (3-ADON), 15-acetyldeoxynivalenol (15-ADON),
nivalenol (NIV) and fusarenon-X (FX). Although cytotoxic effects of individual
mycotoxins have been widely studied, data on combined toxic effects of
mycotoxins are limited. The aim of this study was to assess interactions that occur
in situations of co-exposure to type B trichothecenes. Proliferating Caco-2 cells
were exposed to increasing doses of type B trichothecenes, alone or in binary or
ternary mixture. MTT and Neutral red uptake, respectively linked to mitochondrial
and lysosomial integrities, were used for measurement of intestinal epithelial cell
viability. The five tested mycotoxins had a dose-dependent effect on proliferating
enterocytes and could be classified in increasing order of toxicity: 3-ADON