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SESSION 6: FUTURE CHALLENGE FOR EUROPE AND WORLDWIDE
P141 - Digestibility and absorption of deoxynivalenol-
3-β-glucoside in in vitro models
M. de Nijs, H. van den Top, L. Portier, G. Oegema, E. Kramer, H. van
Egmond, R. Hoogenboom
RIKILT Wageningen UR, PO Box 230, NL-6700AE Wageningen, The Netherlands
E-mail: monique.denijs@wur.nl
Transformation of mycotoxins into glucosides is one of the defences of plants
against toxic effects of mycotoxins. If these masked-mycotoxins are hydrolysed
into their parent compounds in the gastro- intestinal tract (GI-tract), exposure
might be increased. Aim of the study was to determine possible transformation
and translocation of 3-β-glucoside (D3G) and deoxynivalenol (DON) using two in
vitro models.
The fed in vitro digestion model was used to study transformation in the upper GI
tract. Samples of baby food were spiked with DON, D3G or left blank. The in vitro
intestinal absorption model with Caco-2 cells seeded in Transwells was used to
study transformation and translocation. DON, D3G or no mycotoxin (blank) was
added to the apical side of the Caco-2 cell layer, while only blank medium was
added to the basolateral side. Samples were taken from both compartments after
0, 4 and 24 hours. Concentrations of DON, D3G and DOM-1 were analysed in the
chyme and in all transwell samples by LC-ESI-MS/MS. 13 C15-DON standard
solution was added as internal standard in the digestion samples.
No evidence was found in the in vitro experiments for significant elevated
exposure of humans to DON from dietary D3G, since D3G was not hydrolysed to
DON in the digestion model representing the upper part of the GI-tract and D3G
was not hydrolysed to DON by the intestinal epithelial Caco-2 cells. Bioavailability
of D3G in humans may be low as compared to DON since Caco-2 cells did not
absorb D3G, in contrast to DON.
Keywords: deoxynivalenol-3-β-glucoside, translocation, transformation, masked
mycotoxins
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