EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
SESSION 6: FUTURE CHALLENGE FOR EUROPE AND WORLDWIDE<br />
P140 - Degradation <strong>of</strong> fumonisins by microorganisms<br />
in moist corn grain silages<br />
C. Martinez Tuppia 1,2 , C. Barreau 1 , F. Richard-Forget 1 , H. Durand 2 , J. M.<br />
Savoie 1<br />
1 INRA, UR1264 MycSA, Mycology & Food Safety, CS20032, F-33882 Villenave d’Ornon Cedex,<br />
France; 2 Lallemand SAS, Department Animal Nutrition, 3F- 1702 Blagnac Cedex, France<br />
E-mail: ccori.martinez@bordeaux.inra.fr<br />
Fumonisins are toxic secondary metabolites produced by Fusarium spp. on<br />
maize. Unfortunately, fumonisins are highly stable molecules and their<br />
detoxification is poorly suitable for industrial processing. Considering the<br />
worldwide contamination <strong>of</strong> corn by Fusarium species in the field, and that<br />
fumonisins are produced before harvesting, one promising option for reducing the<br />
mycotoxic risk could be the degradation <strong>of</strong> fumonisins by using antagonist<br />
microorganisms isolated directly from plant microbiota before feeding livestock.<br />
Moreover, experimental evidence showed a fumonisins level decrease in some<br />
analysed samples during the moist corn grain silage fermentation. Therefore, the<br />
aim <strong>of</strong> this work is to select an endemic microbial consortium <strong>of</strong> moist corn grain<br />
silage that could be able to transform fumonisins in non-toxic products.<br />
To address this objective, functional screening using classical methods and highresolution<br />
metagenomics approaches will be performed. To achieve our goals,<br />
three main questions will be answered.<br />
1- Are fumonisins actually degraded and detoxified or only adsorbed and not<br />
available for conventional analyses methods? Analytical methods combining<br />
various extractions procedures and fine HPLC-MS analyses <strong>of</strong> the mycotoxins<br />
and their degradation products are under development.<br />
2- Are there any differences in microbial community composition in moist corn<br />
grain silages leading or not to significant fumonisin degradation? Comparison <strong>of</strong><br />
the microbial consortia and metagenomes <strong>of</strong> contrasted moist corn grain silages<br />
samples at different time is a valuable source <strong>of</strong> data on this unexplored microbial<br />
ecosystem and <strong>of</strong> degrading inocula.<br />
3- What are the detoxification mechanisms and the microbial activities involved? It<br />
will be expected to identify “detoxification genes” to be used as markers <strong>of</strong><br />
detoxifying silages that could be used as a predictive and a screening tool to<br />
target degrading microbes.<br />
Keywords: fumonisin, biodegradation, silage<br />
234