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EFS12- Book of abstracts - Contact

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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS<br />

P132 - Study <strong>of</strong> the effect <strong>of</strong> Pseudomonas spp.<br />

fluorescent for the suppression <strong>of</strong> Fusarium wilt <strong>of</strong><br />

tomato<br />

F. Bensaid 1 , M. Benchaabane 2<br />

1 ENSA Elharrach Alger, Algeria; 2 University saad dahlab Blida, Algeria<br />

E-mail: b.fatiha79@yahoo.fr<br />

The objective <strong>of</strong> our present work is the description <strong>of</strong> the antagonistic capacities<br />

<strong>of</strong> two strains <strong>of</strong> Pseudomonas fluorescence (S20 and CHAO) and the<br />

nonpathogenic fungic isolate FO47 Fusarium oxysporum against<br />

phytopathogenics agents: Fusarium oxysporum f.sp lycopersici<br />

Assays in vitro showed that our antagonists inhibit with a high level the mycelia<br />

growth, conidia germination and elongation <strong>of</strong> structure’s pathogens. It has been<br />

noted the implication <strong>of</strong> antibiosis effects as those <strong>of</strong> the trophic competition. The<br />

inhibition <strong>of</strong> these vital parameters, for the development <strong>of</strong> pathogenic, can<br />

contribute amply in the repression <strong>of</strong> its reproduction and infection processes. The<br />

interactions experimented in hosts plants tomato or flax (in situ assays), with the<br />

application <strong>of</strong> the antagonists, permitted to reduce meaningful way the<br />

development <strong>of</strong> both wilt fusarium diseases qualitatively and quantitatively. The<br />

rates <strong>of</strong> infected plants regressed significantly and severity was low.<br />

Soil microbial balance, between the antagonistic population and that <strong>of</strong><br />

pathogenic, can be modulated through microbiological variations or abiotics<br />

additives influencing directly or indirectly the metabolic behavior microbial. In this<br />

experiment, addition <strong>of</strong> glucose or EDTA, could increase or decrease the<br />

resistance <strong>of</strong> soil by activation <strong>of</strong> pathogenic or antagonists, as a result <strong>of</strong><br />

modification and modulation in their metabolic activities. In our tests it was noted<br />

the positive or negative effect on the pathogenic population, expressed through its<br />

infectious capacities, by supporting its development or that <strong>of</strong> the antagonistic<br />

bacteria by stimulating its secondary metabolic activity (synthesis <strong>of</strong><br />

siderophores).<br />

Keywords: Pseudomonas spp. fluorescents, nonpathogenic Fusarium oxysporum,<br />

Fusarium wilt, antagonism, biological - control, induced systemic resistance, soil<br />

receptivity<br />

226

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