EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS<br />
P131 - Identification <strong>of</strong> Pseudomonas bacteria<br />
associated with roots <strong>of</strong> Piper tuberculatum able to<br />
inhibit in vitro growth <strong>of</strong> Fusarium solani f. sp. piperis<br />
A. M. Lima, S. B. Nascimento, W. U. Brito, C. M. Y. Cardoso, S. P. Reis, C. R.<br />
B. Souza<br />
Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará,<br />
Belém, PA, Brazil. 66075-110.<br />
E-mail: bsouza@ufpa.br<br />
Endophytic bacteria have been found colonizing internal tissues in many different<br />
plants, where they can promote several beneficial effects, including defense<br />
against pathogens. In this work we aimed to identify endophytic bacteria<br />
associated with roots <strong>of</strong> the tropical Piper tuberculatum, which is known for its<br />
resistance to infection by Fusarium solani f. sp. piperis, causal agent <strong>of</strong> root rot<br />
disease <strong>of</strong> black pepper (Piper nigrum L.) in the Amazon region. According to<br />
comparative analysis <strong>of</strong> 16S rRNA gene sequences, we isolated 23 endophytes<br />
(Pt1 to Pt23) belonging to 13 genera: Bacillus, Paenibacillus, Pseudomonas,<br />
Enterobacter, Rhizobium, Sinorhizobium, Agrobacterium, Ralstonia, Serratia,<br />
Cupriavidus, Mitsuaria, Pantoea, and Staphylococcus. Antagonistic assays<br />
revealed that Pt12 and Pt13 isolates, identified as Pseudomonas putida and<br />
Pseudomonas sp., respectively, were able to inhibit the F. solani f. sp. piperis<br />
growth on PDA (potato dextrose agar) medium. Statistical analyzes using Tukey<br />
test showed that Pt12 and Pt13 isolates significantly inhibited the F. solani f. sp.<br />
piperis growth in 38.96% and 55.31%, respectively. Here, we report the first<br />
evidence <strong>of</strong> P. tuberculatum associated endophytic bacteria as potential biological<br />
control agent <strong>of</strong> pathogen causing the root rot disease <strong>of</strong> black pepper in the<br />
Amazon region.<br />
Supported by: CNPq, Capes, FAPESPA, UFPA<br />
Keywords: Amazon region, Biological control, Black pepper, Endophytic bacteria,<br />
Fusarium solani f. sp. piperis, Root rot disease.<br />
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