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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS P131 - Identification of Pseudomonas bacteria associated with roots of Piper tuberculatum able to inhibit in vitro growth of Fusarium solani f. sp. piperis A. M. Lima, S. B. Nascimento, W. U. Brito, C. M. Y. Cardoso, S. P. Reis, C. R. B. Souza Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, Brazil. 66075-110. E-mail: bsouza@ufpa.br Endophytic bacteria have been found colonizing internal tissues in many different plants, where they can promote several beneficial effects, including defense against pathogens. In this work we aimed to identify endophytic bacteria associated with roots of the tropical Piper tuberculatum, which is known for its resistance to infection by Fusarium solani f. sp. piperis, causal agent of root rot disease of black pepper (Piper nigrum L.) in the Amazon region. According to comparative analysis of 16S rRNA gene sequences, we isolated 23 endophytes (Pt1 to Pt23) belonging to 13 genera: Bacillus, Paenibacillus, Pseudomonas, Enterobacter, Rhizobium, Sinorhizobium, Agrobacterium, Ralstonia, Serratia, Cupriavidus, Mitsuaria, Pantoea, and Staphylococcus. Antagonistic assays revealed that Pt12 and Pt13 isolates, identified as Pseudomonas putida and Pseudomonas sp., respectively, were able to inhibit the F. solani f. sp. piperis growth on PDA (potato dextrose agar) medium. Statistical analyzes using Tukey test showed that Pt12 and Pt13 isolates significantly inhibited the F. solani f. sp. piperis growth in 38.96% and 55.31%, respectively. Here, we report the first evidence of P. tuberculatum associated endophytic bacteria as potential biological control agent of pathogen causing the root rot disease of black pepper in the Amazon region. Supported by: CNPq, Capes, FAPESPA, UFPA Keywords: Amazon region, Biological control, Black pepper, Endophytic bacteria, Fusarium solani f. sp. piperis, Root rot disease. 225
SESSION 5: DISEASE CONTROL AND FORECASTING MODELS P132 - Study of the effect of Pseudomonas spp. fluorescent for the suppression of Fusarium wilt of tomato F. Bensaid 1 , M. Benchaabane 2 1 ENSA Elharrach Alger, Algeria; 2 University saad dahlab Blida, Algeria E-mail: b.fatiha79@yahoo.fr The objective of our present work is the description of the antagonistic capacities of two strains of Pseudomonas fluorescence (S20 and CHAO) and the nonpathogenic fungic isolate FO47 Fusarium oxysporum against phytopathogenics agents: Fusarium oxysporum f.sp lycopersici Assays in vitro showed that our antagonists inhibit with a high level the mycelia growth, conidia germination and elongation of structure’s pathogens. It has been noted the implication of antibiosis effects as those of the trophic competition. The inhibition of these vital parameters, for the development of pathogenic, can contribute amply in the repression of its reproduction and infection processes. The interactions experimented in hosts plants tomato or flax (in situ assays), with the application of the antagonists, permitted to reduce meaningful way the development of both wilt fusarium diseases qualitatively and quantitatively. The rates of infected plants regressed significantly and severity was low. Soil microbial balance, between the antagonistic population and that of pathogenic, can be modulated through microbiological variations or abiotics additives influencing directly or indirectly the metabolic behavior microbial. In this experiment, addition of glucose or EDTA, could increase or decrease the resistance of soil by activation of pathogenic or antagonists, as a result of modification and modulation in their metabolic activities. In our tests it was noted the positive or negative effect on the pathogenic population, expressed through its infectious capacities, by supporting its development or that of the antagonistic bacteria by stimulating its secondary metabolic activity (synthesis of siderophores). Keywords: Pseudomonas spp. fluorescents, nonpathogenic Fusarium oxysporum, Fusarium wilt, antagonism, biological - control, induced systemic resistance, soil receptivity 226
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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Page 241 and 242: de Hoog G. S., 89, 114, 236 de Kler
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Page 247 and 248: BOKU-University of Natural Resource
Page 249 and 250: touatisihem03@yahoo.fr He Xinyao In
Page 251 and 252: steve.oliver@bioc.cam.ac. uk Ortega
Page 253 and 254: Subramaniam Gopal Agriculture and A
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