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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS P127 - Biological formulations for control of Fusarium verticillioides and fumonisins in maize at field level M. Sartori 3 , A. Nesci 1, 2 , M. Etcheverry 1,2 1 Laboratorio de Ecología Microbiana. Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas Físico Químicas y Naturales. Universidad Nacional de Río Cuarto, Ruta Nacional N° 36 Km 601, Río Cuarto (5800), Córdoba, Argentina; 2 Members of the Research Career, Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina; 3 Doctoral Fellow of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Argentina E-mail: metcheverry@exa.unrc.edu.ar Fusarium verticillioides (Sacc.) Nirenberg is a seed-transmitted pathogen that infects maize during the different growth and developmental stages. Under favorable conditions, the pathogen produces fumonisins that have potencial toxicity for humans and animals. In order to control this fungal species different strategies are being considered. Biological control with antagonistics native bacteria, Bacillus amyloliquefaciens and Microbacterium oleovorans against F. verticillioides infection and fumonisin B1 production in field-grown was evaluated. During four consecutive maize growing seasons (2004-2005; 2005-2006; 2006- 2007 and 2007-2008) the biocontrol agents were applied in liquid inoculum, and in the 2009-2010 growing seasons the biocontrol agents were applied as the active components of two liofilized formulations. All assays were performed in Río Cuarto, Córdoba province, central area of Argentina. Inoculation with liquid inoculum of both biocontrol agents significantly reduced F. verticillioides propagules in maize grains and promoted a significant reduction on fumonisin B1 contents of harvested grains during three of the four evaluated season. In 2007- 2008 growing season the reduction were of 47% and 81% with B. amyloliquefaciens and M. oleovorans, respectively. Inoculation with liofilized formulations did not affect F. verticillioides propagules, but fumonisin B1 in harvested grains were significantly reduced. In 2009-2010 growing season with the formulations of B. amyloliquefaciens the reduction was 51% and with formulations of M. oleovorans was 72%. Therefore, the addition of biocontrol agents could significantly improve the quality of maize, and also offers the advantage of working with a biological product that does not harm the environment. The liquid inoculum has a limited useful life, and therefore the inoculation of the seeds must be performed in a short time. The liofilized formulations maintain the biocontrol activity of the bacterial agents, and they also have the advantage of being stable both to prolonged storage and to environmental fluctuations in the field. Keywords: Fusarium verticillioides, fumonisin B1, maize, field biocontrol 221
SESSION 5: DISEASE CONTROL AND FORECASTING MODELS P128 - Lactic acid bacteria [LAB]: potential for control of Fusarium growth N. Dalanaj 1,2 , F. Gaggìa 2 , L. Baffoni 2 , D. Alkadri 2 , P. Nipoti 2 , D. Di Gioia 2 1 Department of Industrial chemistry, Faculty of Natural Sciences, Tirana University. Bulevardi Zogu i Parë,Tirana; 2 Department of Agricultural Sciences - DipSA, Bologna University, Viale Fanin 44, 40127 Bologna, Italy E-mail: diana.digioia@unibo.it Lactic Acid Bacteria [LAB] are worldwide known as probiotic microorganisms. One of the most important probiotic feature is their strong activity against a wide range of harmful bacteria/fungi (Gaggìa et al., 2011). Production of organic acids and production of antagonistic compounds are the main mechanisms described nowadays. This work focuses on the in vitro selection of LAB strains isolated from environmental sources against Fusarium genus. Fusarium is a well distributed genus of filamentous fungi affecting plant, animal and human health. In the agricultural field, Fusarium is one of the most important plant pathogenic genera, with a record of devastating infections in various economically important plants. In this study, in vitro antagonistic effects of 18 isolates of Lactobacillus spp. were evaluated against F. oxysporum f. sp. lactucae, agent of tracheofusariosis in Lactuca sativa, F. culmorum and F. graminearum, agents of severe diseases in cereals. Fungal inhibition was performed using spot agar test in a triplicate assay (Magnusson et al., 2003). Overnight cultures of LAB were inoculated as spot on MRS agar plates and allowed to grow at 30°C for 24 h. The plates were then overlaid with 10 ml of PDA containing 10 4 mould spores/ml. After 48-72 h of aerobic incubation at 25°C, the zone of inhibition was measured. All LAB strains (except one) showed antifungal activity against all Fusarium species (inhibition halo between 23.7- 65.3 mm). The highest halos were obtained against F. graminearum on which 50% of the LAB strains showed a total growth inhibition of the colony. These results are probably related to the high amount of organic acids produced by LAB strains but it can also be hypothesized the presence of antifungal compounds. In vivo application of the most successful LAB strains is foreseen towards wheat and lettuce to confirm in vitro results. Keywords: LAB, biocontrol, Fusarium, growth 222
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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Page 241 and 242: de Hoog G. S., 89, 114, 236 de Kler
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Page 247 and 248: BOKU-University of Natural Resource
Page 249 and 250: touatisihem03@yahoo.fr He Xinyao In
Page 251 and 252: steve.oliver@bioc.cam.ac. uk Ortega
Page 253 and 254: Subramaniam Gopal Agriculture and A
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