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EFS12- Book of abstracts - Contact

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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS<br />

P122 - Distribution <strong>of</strong> the airborne inoculum <strong>of</strong><br />

Gibberella zeae in Belgium<br />

G. Dedeurwaerder 1 , M. Duvivier 2 , P. Hellin 1 , M.-E. Renard 1 , V. Van Hese 1 , A.<br />

Legrève 1<br />

1 Université catholique de Louvain – Earth and Life Institute, Applied Microbiology, Phytopathology,<br />

Croix du Sud 2, box L7.05.03, B-1348 Louvain-la-Neuve ; 2 Walloon Agricultural Research Centre,<br />

Plant Protection and Ecotoxicology Unit, Rue du Bordia 11, B-5030 Gembloux, Belgium<br />

Email: geraldine.dedeurwaerder@uclouvain.be<br />

Fusarium head blight (FHB) is a common fungal disease in winter wheat in<br />

Belgium, causing yield losses and sanitary problems due to the production <strong>of</strong><br />

mycotoxins by species associated with the disease. Fusarium graminearum<br />

(teleomorph Gibberalle zeae), one <strong>of</strong> the most important species involved in the<br />

species complex causing FHB, is able to produce wind-dispersed ascospores by<br />

sexual reproduction. In order to analyse the distribution <strong>of</strong> G. zeae airborne<br />

inoculum throughout the year, particularly between heading and flowering, and to<br />

understand the role <strong>of</strong> this inoculum in the infection <strong>of</strong> the wheat ears, a network<br />

<strong>of</strong> Burkard spore traps was set up in fields in the Walloon region in Belgium in the<br />

2011-2012 growing season. Total DNA from each fragment <strong>of</strong> spore trap tape,<br />

corresponding to 1 day <strong>of</strong> sampling, was extracted and the quantity <strong>of</strong> G. zeae<br />

was assessed using a real-time polymerase chain reaction (PCR) assay. Initial<br />

results showed the occurrence <strong>of</strong> G. zeae airborne inoculum between heading<br />

and flowering. The relationship between the distribution <strong>of</strong> the inoculum and the<br />

prevalence <strong>of</strong> G. zeae on infected ears collected in fields was studied in order to<br />

assess whether spore traps, coupled with real-time PCR, could be used to<br />

improve the understanding <strong>of</strong> FHB epidemiology, the prediction <strong>of</strong> this disease<br />

and the control strategies.<br />

Keywords: Fusarium head blight, spore trap, wheat<br />

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