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SESSION 5: DISEASE CONTROL AND FORECASTING MODELS
P121 - Pathogenic fungi associated with Fusarium
seedling root rot in winter cereals in 2012
A. Jonaviciene, R. Semaskiene, S. Suproniene
Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, Instituto aleja 1,
Akademija, LT 58344, Kedainiai distr., Lithuania
E-mail: akvile@lzi.lt
In order to establish the species composition of seedling root rot in winter cereals
in Lithuania laboratory and field experiments were done in winter rye ‘Agronom H‘,
winter barley ‘Cinderella‘ and winter wheat ‘Ada‘ at Institute of Agriculture, LRCAF
in 2012. The seeds heavily infected with Fusarium spp. were drilled in the field.
Samples of 400 seeds of each crop were examined on a PDA medium. Infection
level of Fusarium pathogens on the seeds of winter triticale was 42 %, of winter
barley – 38 % and of winter wheat – 45 %. Seedlings with brown spots on the
coleoptiles were collected from untreated plots at BBCH 14-21 growth stages.
Twenty plants per plot were taken from 4 replicates. Samples of plant stem
fragments with symptoms (1-1.5 cm) were washed for 20 min in running water,
surface – sterilized in 1.5 % sodium hypochlorite for 2 min, washed 3 times in
sterile water ant plated onto a PDA medium with 150 mg l -1 streptomycin sulphate
(Irzykowska, Baturo, 2008; Kwaśna et al. 1991). After 10 days of incubation at
20°C, Fusarium spp. was transferred to Petri dishes with PDA and SNA media for
10 days at 23°C and identified according to the manuals of Nelson et al. (1983)
and Leslie and Summerell (2006). F. culmorum, F. avenaceum and F.
graminerum dominated on seedling stem base of winter rye, F. avenaceum, F.
tricinctum and F. graminearum – on winter barley. F. culmorum, F. tricinctum were
identified on winter wheat seedlings.
Acknowledgements: The paper presents research findings obtained through the
long-term research programme "Harmful organisms in agro and forest
ecosystems" implemented by Lithuanian Research Centre for Agriculture and
Forestry.
Keywords: Fusarium, root rot, infection, identification
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