EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P106 - Genetic variation for ear rot resistance and<br />
mycotoxin content <strong>of</strong> Polish maize elite inbreed lines<br />
after inoculation with Fusarium graminearum and F.<br />
verticillioides<br />
E. Czembor 1 , A. Waśkiewicz 2 , Ł. Stępień 3<br />
1 Plant Breeding and Acclimatization Institute – NRI, Radzikow, 05-870 Blonie, Poland; 2 Department <strong>of</strong><br />
Chemistry, Poznan University <strong>of</strong> Life Sciences, Wojska Polskiego 75, 60-625 Poznań, Poland;<br />
3 Department <strong>of</strong> Pathogen Genetics and Plant Resistance, Institute <strong>of</strong> Plant Genetics, Polish Academy<br />
<strong>of</strong> Sciences, Strzeszyńska 34, 60-479 Poznań, Poland<br />
E-mail: e.czembor@ihar.edu.pl<br />
Poland is the fifth European producing country <strong>of</strong> maize, with an increasing area.<br />
Ear rots caused by Fusarium are important diseases affecting yield and causing<br />
grain mycotoxin contamination. This country has variable weather conditions –<br />
with big differences between regions and years. It is influenced by a mild oceanic<br />
climate from the west and a dry continental climate from the east. Because <strong>of</strong> this<br />
Fusarium graminearum and F. verticillioides are two ear rotting species commonly<br />
connected with maize kernel samples and their prevalence depends on<br />
environmental conditions. Inbreed lines which are commonly used in Polish<br />
breeding programmes belong mostly to 2 distinct genetic categories: flint and<br />
dent. However, lines from Lancaster, IDT and SSS groups are also introduced.<br />
One hundred inbreed lines were evaluated across 2011 – 2012. Ear rot resistance<br />
was determined separately after inoculatation with F. graminearum and F.<br />
verticillioides using tooth-picks and under natural infection. Disease severity and<br />
selection pressure after inoculation with F. graminearum was higher than after<br />
inoculation with F. verticillioides. Lancaster, IDT, SSS and SSS/IDT groups were<br />
characterized as a most susceptible. Under natural infection differences between<br />
inbreeds were also significant. Based on the obtained results group <strong>of</strong> the most<br />
resistant and most susceptible genotypes were taken to determine mycotoxin<br />
content in the kernel samples and rachis fractions (DON, ZEA and FBs) using<br />
HPLC method. The correlation coefficients between toxin content and disease<br />
scores were highly significant. Additionally the presence <strong>of</strong> Fusarium species,<br />
which colonized kernels under natural infection was determined (based on the<br />
SCAR-PCR markers and the translation elongation factor (tef-1alpha) sequences<br />
analyses). F. verticillioides was the prevalent species (>75% <strong>of</strong> samples tested<br />
contained this pathogen). The ability <strong>of</strong> F. proliferatum and F. verticillioides to<br />
produce fumonisins was confirmed by the identifiaction <strong>of</strong> the FUM1 gene<br />
presence.<br />
Keywords: ear rot resistance, maize inbreed lines, toxin content, sources <strong>of</strong><br />
resistance<br />
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