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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P102 - Transient silencing of a PR-4 gene decreases type I resistance against Fusarium graminearum J. J. Peter, G. Siegwart, W. Schweiger, B. Steiner and H. Buerstmayr BOKU - University of Natural Resources and Life Sciences, A-1180 ViennaDepartment for Biotechnology in Plant production, A-3430 Tulln E-mail: gerald.siegwart@boku.ac.at Pathogenesis-related proteins (PR) are part of the complex defense arsenal of plants against a wide range of pathogens. Among them members of the class 4 proteins (PR-4) have the capability inhibiting the germination of Fusarium culmorum and also Fusarium graminearum spores (Caruso et al. 1996). We have investigated the transcriptomic response of the highly resistant wheat line CM- 82036, a derivative of Sumai3, to infection with F. graminearum and identified a novel PR-4 gene highly upregulated in response to the pathogen (250-fold). This gene is unrelated to the two prominent resistance QTL Fhb1 and Qfhs.ifa-5A encoded by CM-82036, as it remains significantly changed after statistically reducing the CM-82036 response to the response of a near-isogenic line harboring both QTL in a susceptible background. Similarly we identified a WIR1 gene, which is also highly upregulated (>60-fold) for the non-Fhb1/Qfhs.ifa-5A related background of CM-82036. We have cloned these genes and performed virus-induced gene silencing (VIGS) to see whether transiently silencing has a negative impact on the high resistance of CM-82036. We observed a significantly faster initial infection of spikelets within the first days after inoculation with F. graminearum spores in plants with the repressed PR-4. Similarly we tested tested WIR1 for which initial test results also look promising. Detailed results will be presented. Keywords: PR, virus induced gene silencing, WIR1, wheat 195
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P103 - RNA-Sequencing as a tool for the analysis of the pathosystem maize-Fusarium verticillioides A. Lanubile, V. Maschietto, A. Marocco Institute of Agronomy, Genetics and Field Crops, University Cattolica del Sacro Cuore, Via Emilia Parmense 84, 29122 Piacenza, Italy E-mail: alessandra.lanubile@unicatt.it Fusarium verticillioides is the causal agent of Fusarium ear rot in maize and contaminates the grain with fumonisins, a family of mycotoxins that affects feed and food. Concern is increasing about the fumonisins because of emerginig evidences of their involvement in several human and animal diseases. To clarify the molecular processes undergoing in maize upon infection, the RNA- Seq technology has been applied to characterize the expression profile of resistant and susceptible kernels of maize 72 hours after F. verticillioides infection. More than 100 million sequence reads were generated for condition (infected/uninfected). The sequence reads were analyzed to measure gene expression levels, to detect alternative splicing events and single nucleotide polymorphisms. We observed 2,006 and 2,629 differentially expressed genes 72 hours after infection for the resistant and susceptible maize genotypes, respectively, of which 927 were in common and showed 5,342 SNPs variants. About 320,000 and 175,000 reads mapped on Fusarium genome in the susceptible and resistant genotypes, respectively, and 129 fungal genes were differentially regulated in both lines. This exhaustive overview of gene expression dynamics demonstrates the utility of RNA-Seq for identifying single nucleotide polymorphisms and describing how plant transcriptomes change during pathogen infection. The identification of differentially expressed plant genes that interact with fungus will produce useful tools for the identification of candidate genes, the development of molecular markers and their use for selection of resistant maize genotypes by means of marker assisted selection. Keywords: RNA-Seq, candidate genes, Fusarium verticillioides, Zea mays 196
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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Page 241 and 242: de Hoog G. S., 89, 114, 236 de Kler
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Page 247 and 248: BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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