EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P102 - Transient silencing <strong>of</strong> a PR-4 gene decreases<br />
type I resistance against Fusarium graminearum<br />
J. J. Peter, G. Siegwart, W. Schweiger, B. Steiner and H. Buerstmayr<br />
BOKU - University <strong>of</strong> Natural Resources and Life Sciences, A-1180 ViennaDepartment for<br />
Biotechnology in Plant production, A-3430 Tulln<br />
E-mail: gerald.siegwart@boku.ac.at<br />
Pathogenesis-related proteins (PR) are part <strong>of</strong> the complex defense arsenal <strong>of</strong><br />
plants against a wide range <strong>of</strong> pathogens. Among them members <strong>of</strong> the class 4<br />
proteins (PR-4) have the capability inhibiting the germination <strong>of</strong> Fusarium<br />
culmorum and also Fusarium graminearum spores (Caruso et al. 1996). We have<br />
investigated the transcriptomic response <strong>of</strong> the highly resistant wheat line CM-<br />
82036, a derivative <strong>of</strong> Sumai3, to infection with F. graminearum and identified a<br />
novel PR-4 gene highly upregulated in response to the pathogen (250-fold). This<br />
gene is unrelated to the two prominent resistance QTL Fhb1 and Qfhs.ifa-5A<br />
encoded by CM-82036, as it remains significantly changed after statistically<br />
reducing the CM-82036 response to the response <strong>of</strong> a near-isogenic line<br />
harboring both QTL in a susceptible background. Similarly we identified a WIR1<br />
gene, which is also highly upregulated (>60-fold) for the non-Fhb1/Qfhs.ifa-5A<br />
related background <strong>of</strong> CM-82036. We have cloned these genes and performed<br />
virus-induced gene silencing (VIGS) to see whether transiently silencing has a<br />
negative impact on the high resistance <strong>of</strong> CM-82036. We observed a significantly<br />
faster initial infection <strong>of</strong> spikelets within the first days after inoculation with F.<br />
graminearum spores in plants with the repressed PR-4. Similarly we tested tested<br />
WIR1 for which initial test results also look promising. Detailed results will be<br />
presented.<br />
Keywords: PR, virus induced gene silencing, WIR1, wheat<br />
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