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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P98 - Impact of co-infection by Microdochium spp. and Fusarium graminearum on the assessment of new wheat varieties’ tolerance to FHB and deoxynivalenol B. Méléard 1 , E. Gourdain 1 , M. Gauthier 1 , P. Du Cheyron 2 1 Service Qualités-Valorisations, ARVALIS - Institut du végétal, Station Expérimentale, 91720 Boigneville, France; 2 Service Génétique, Physiologie et Protection des Plantes, ARVALIS - Institut du végétal, IBP – Université Paris Sud, Rue de Noetzlin – Bât. 630, 91405 Orsay cedex, France E-mail: B.MELEARD@arvalisinstitutduvegetal.fr By choosing the variety to produce, a farmer has a good mean to manage the Fusarium Head Blight (FHB) risk and their associated toxins. Many efforts have been made by breeders since a few years in France to improve tolerance of new varieties to FHB, mostly for F.graminearum. Also the assessment of new varieties on this criterion is a major stake. For this reason the French registration system (Comité Technique Permanent de la Sélection) has developed specific trials by using maize debris spread on soil and water spraying. New varieties are evaluated by a notation from very sensitive to tolerant. Sometimes the observations reveal the presence of both F.graminearum and Microdochium fungus in the trials. In this case the question is to know if such a situation does impact the ranking of varieties according to the ratio between F.graminearum and Microdochium on the grain by the way of a difference in the production of toxins. A multilocal trials network has been used in a collaborative research project named ECOFUSA with the financial support of CASDAR (Compte d’Affectation Spéciale pour le Développement Agricole et Rural). Neuf varieties have been characterised for the presence of F. graminearum and Microdochium by the mean of visual notation, quantification of fungus and Deoxynivalenol (DON) content in grain. For the statistical treatment trials have been classified in 3 groups according to the ratio between F. graminearum and Microdochium on the grain. Data have been analysed by a mixed model taking into account the following factors, variety, precocity, location x year and Fusarium/Microdochium ratio. Differences in the ranking of varieties for DON content in the trials are non-significant. This preliminary study that needs to be continued seems to show that a trial where the rate of Microdochium compared to F. graminearum is high (until 50%) could be used to classify varieties on their susceptibility to DON accumulation. Keywords: FHB, DON, varieties, co-infection 191
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P99 - Testing varieties at GEVES for resistance to Fusarium head blight on cereals: A way to improve genetic progress in the French Catalogue and to reduce the use of pesticides V. Cadot 1 , J. P. Maigniel 2 GEVES (Groupe d’Etude et de contrôle des Variétés Et des Semences- French Group for the Study and Inspection of Varieties and Seeds), 1 25 Rue Georges Morel - CS 90024 - 49071 BEAUCOUZE Cedex – France, 2 Domaine de l'Anjouère - 49370 LA POUEZE- France E-mail: valerie.cadot@geves.fr Testing candidate varieties resistance to pests and diseases for registration in the French catalogue is very important for varietal innovation, as it provides a description of resistant varieties to the farmers and could also encourage breeders to improve the level of disease resistance; GEVES being responsible for carrying out these studies. Regarding cereal diseases,resistant cultivars are advantaged by bonus points in the final cotation of Value for Cultivation, Use and Sustainability, in order to facilitate their registration. For Fusarium Head Blight, a bonus is accorded for a cotation superior or equal to 6 and a penalty is given for a cotation inferior or equal to 3, with the resistance cotation from 1: susceptible to 9: resistant. FHB resistance is assessed over a two year period in a specific CTPS network, composed of 7 winter wheat trials, with twenty controls shared in 4 groups of earliness. The trials were contaminated, either by corn stubbles with Fusarium graminearum, or by spraying a mixture of F.culmorum and F. graminearum; strains being chosen in accordance with their toxinogenocity for deoxynivalenol (DON) and aggressiveness. From 2008 to 2012, the evolution of the resistance level to Fusarium of 150 registered cultivars was studied, with about 30 registered cultivars per year out of 80 applicants. The amplitude of the resistance cotation goes from 2.5 to 6.5 and the median is worth 4.4. A genetic progress of the resistance level was observed, with a significant reduction of the susceptible class (from 31% cultivars to 9%), an important increase of the intermediate class (from 53% to 71%) and a slight increase of the resistant class (from 16 % to 21%). Integration of DON into the CTPS is currently being considered. Keywords: Fusarium, wheat, registration, resistance 192
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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Page 241 and 242: de Hoog G. S., 89, 114, 236 de Kler
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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