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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P96 - Oat resistance to HT2 and T2-producing Fusarium langsethiae T. Stancic 1 , S. Cowan 2 , C. Howarth 2 , S. Edwards 1 1 Harper Adams University, Newport, Shropshire, TF10 8NB, UK - 2 IBERS, Aberystwyth University, Plas Gogerddan, Aberystwyth, SY23 3EB E-mail: tstancic@harper-adams.ac.uk Fusarium langsethiae, a species which was first described in 2004, is known to be the predominant mycotoxin producing species on oats (Avena sativa L.) in the UK and results in contamination of the grain with the trichothecene mycotoxins HT2 and T2. The European Commission is setting indicative limits for the combined concentration of HT2 and T2 (HT2+T2) in food and feed. In observational studies across the UK between 2002 and 2008 around 16% of samples collected at harvest exceeded the proposed investigative limit of 1000 µg/kg HT2+T2 for unprocessed oats intended for human consumption. Winter variety trials tended to have higher levels of HT2+T2 compared to spring variety trials. It is not clear whether the difference observed is due to agronomic (i.e. drilling date) or genetic differences. To test the hypothesis that the difference observed were not due to agronomy, six spring and six winter varieties were drilled together in randomised block experiments at three sites in the UK in autumn 2011 and in spring 2012. Samples of panicles and grain samples are currently being quantified for HT2 and T2. To test the hypothesis of whether crop height is a resistance trait, samples of panicles were collected from a field trial run by Aberystwyth University of a mapping population developed from a cross between short and tall winter oat varieties (Buffalo and Tardis). Samples are currently being analysed for F. langsethiae DNA and HT2+T2 concentration. Preliminary results indicate that taller lines had a consistently low level of HT2+T2 whereas shorter lines had either a high or a low level of HT2+T2. The Buffalo x Tardis mapping population will be used for the identification of QTL for susceptibility and to determine genetic linkage with other agronomic traits such as height. Keywords: Fusarium langsethiae, HT2, T2, oat 189
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P97 - Susceptibility of cereal species to Fusarium langsethiae, a potent producer of HT2 and T2 S. Edwards, N. Opoku, M. Back Harper Adams University, Newport, Shropshire. TF10 8NB, UK E-mail: sedwards@harper-adams.ac.uk A field survey was performed to study Fusarium langsethiae in wheat, barley and oats in commercial UK crops (2009 – 2011). Plants sampled (from tillering to harvest) were divided into roots, leaves, lower stem, upper stem and inflorescence/head sub-samples depending on the growth stage of the cereal. F. langsethiae DNA was quantified using real-time PCR and fusarium mycotoxins HT2 and T2 were estimated from head samples at harvest using ELISA. Results showed oat to contain the highest levels of both F. langsethiae DNA and HT2+T2 mycotoxins in harvested heads of the cereals studied. The development of F. langsethiae in all three cereals appeared to be similar. Head infection, if it occurred, started at head emergence rather than at flowering. Seemingly symptomless oat heads could have high levels of F. langsethiae DNA and HT2+T2, confirming previous suggestions that F. langsethiae is a symptomless pathogen of oats. To identify if differences observed between cereals were genetic, rather than agronomic, field experiments were conducted with spring and autumn sown cereals (wheat, barley and oats) at two sites. Experiments contained three varieties of each cereal in a split plot design with variety as a sub-plot of cereal in a randomized block design with four blocks. The same agronomy was applied to all plots. F. langsethiae DNA and HT2+T2 were quantified in cereal heads and grains at harvest. Similar results were obtained for all four experiments. There were significantly higher F. langsethiae DNA and HT2+T2 in oats compared to wheat and barley and there were significant differences between oat varieties. Regression analysis of HT2+T2 to F. langsethiae DNA concentration grouped by cereal identified that wheat and barley samples fitted on the same line but a different line existed for oats which had a higher concentration of HT2 and T2 per unit of F. langsethiae DNA. Keywords: langsethiae, HT2, T2, oat 190
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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Page 241 and 242: de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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