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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P94 - Resistance of winter wheat breeding lines to Fusarium head blight and accumulation of Fusarium toxins in grain H. Wiśniewska 1 , T. Góral 2 , P. Ochodzki 2 , D. Walentyn-Góral 2 , J. Belter 1 , M. Kwiatek 1 , J. Bogacki 3 , T. Drzazga 4 , B. Ługowska 3 , P. Matysik 5 , E. Witkowski 6 , K. Rubrycki 7 , U. Woźna–Pawlak 7 1 Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska str., 60-479 Poznan, Poland; 2 Department of Plant Pathology, Plant Breeding and Acclimatization Institute NRI, Radzików, 05-870 Blonie, Poland; 3 Danko Plant Breeders Ltd., Choryń 27,64-000 Kościan, Poland; 4 Małopolska Plant Growing Company – HBP LLC, 4 Zbożowa str., 30-002 Kraków, Poland; 5 Plant Breeding Strzelce Ltd., 20 Główna str., 99-307 Strzelce, Poland; 6 Plant Breeding Smolice Ltd., Smolice 41, 63- 740 Kobylin, Poland; 7 Poznań Plant Breders Ltd., 5 Kasztanowa str., 03-004 Tulce, Poland E-mail: hwis@igr.poznan.pl Fusarium head blight (FHB) is an important cereal disease caused by Fusarium species. Disease is mainly damaging for bread wheat. The aim of this work was (1) to evaluate FHB resistance variability of wheat lines and (2) to analyze concentration of Fusarium toxins in grain. This research was conducted on 71 winter wheat lines differing in genetic background. Cultivars ‘Arina’ and ‘Tonacja’ were used as controls. Lines were sown in two field experiments located in Cerekwica, Western Poland and in Radzików, Central Poland. Field experiments were established as a randomized complete block design. Wheat heads were inoculated at flowering stage with conidial suspension of Fusarium culmorum isolates producing deoxynivalenol (DON), nivalenol (NIV) and zearalenon (ZEA), at a rate of 100 000 spores per mL. The disease was rated 14 and 16 days after inoculation. Fusarium head blight index (FHBi) was calculated. FHBi ranged from 8.3 to 49.9%. Only 3 lines showed infection below 10%. Percentage of Fusarium damaged kernels (FDK) was high, ranging from 50.2% to 98.0%. The FDKs of ‘Tonacja’ and ‘Arina’ cultivars were also high (65.2 and 69.7%). However, the FDK of line ‘DED’ (susceptible check) was 82.2%. Fusarium damaged kernels of only 5 genotypes did not exceed 60.0%. The correlation coefficient of FHBi versus FDK was significant (r=0.575). Grain from 35 lines from two locations, with low FHBi and FDK, was analyzed for the presence of Fusarium toxins. DON amount was the highest, and ranged from 7.041 ppm to 22.651ppm. The total amount of NIV was also high, ranging from 3.331 ppm to 19.285 ppm. The lowest concentration of ZEA was found in ‘Arina’ – 128 ppb, the highest ZEA level was 8432 ppb. Research was supported by the Ministry of Agriculture and Rural Development projects: HOR hn 801 - 13/11 and HOR hn 078-801-9/11. Keywords: Fusarium, mycotoxin, resistance, wheat 187
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P95 - Evaluation of Fusarium Head Bblight resistance in a panel of durum wheat (Triticum turgidum L.) A. Brunazzi 1 , S. Greggio 2 , P. Lancioni 1 , E. Braida 1 , P. Mantovani 1 , R. Bovina 1 , G. Gasparini 1 , S. Tonti 3 , A. Prodi 4 , G. Ferrazzano 1 1 Società Produttori Sementi S.p.A. (PSB), Via Macero 1, 40050 Argelato (BO), Italy; 2 Università degli Studi di Ferrara, Via Paradiso 12, 44121 Ferrara, Italy; 3 Ente Nazionale delle Sementi Elette (ENSE) Via Ca’ Nova Zampieri 37, 37057 San Giovanni Lupatoto (VR), Italy; 4 Dipartimento di Scienze e Tecnologie Agroambientali (DiSTA), Viale Fanin 44, 40127 Bologna, Italy E-mail: a.brunazzi@prosementi.com Fusarium Head Blight (FHB) caused by Fusarium spp. is one of the most destructive fungal disease of wheat; it causes dramatic yield and quality losses and contamination of cereal products with mycotoxins, in particular deoxynivalenol (DON). The release of new varieties resistant to FHB and less affected by mycotoxin contamination, is the most efficient way to contrast this disease, with no additional costs for the farmers and with advantages for the consumers health. In this study, we set up a two year field experiment under artificial inoculation with a mixture of virulent and toxigenic strains of Fusarium culmorum and F. graminearum in Argelato (Bologna), in order to evaluate a panel of durum wheat genotypes (100) for their reaction to FHB; three common wheat varieties know to be resistant to FHB (Sumai 3, Gondo and Heilo) were also included as resistant control. At anthesis, ten spikes per genotype were tagged and harvested separately. FHB incidence and severity were rated on the selected spikes in two different times. We also evaluated: Fusarium Damaged Kernels (FDK), Area Under the Disease Progress Curve (AUDPC), Damage Index (DI) and DON content. The disease incidence and severity data matrixes were analyzed by ANOVA and the analyses revealed the presence of significant difference among the investigated genotypes. Highly significant correlation were detected between DI and AUDPC (r=0.96, p
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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