EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P94 - Resistance <strong>of</strong> winter wheat breeding lines to<br />
Fusarium head blight and accumulation <strong>of</strong> Fusarium<br />
toxins in grain<br />
H. Wiśniewska 1 , T. Góral 2 , P. Ochodzki 2 , D. Walentyn-Góral 2 , J. Belter 1 , M.<br />
Kwiatek 1 , J. Bogacki 3 , T. Drzazga 4 , B. Ługowska 3 , P. Matysik 5 , E. Witkowski 6 ,<br />
K. Rubrycki 7 , U. Woźna–Pawlak 7<br />
1 Institute <strong>of</strong> Plant Genetics, Polish Academy <strong>of</strong> Sciences, 34 Strzeszyńska str., 60-479 Poznan,<br />
Poland; 2 Department <strong>of</strong> Plant Pathology, Plant Breeding and Acclimatization Institute NRI, Radzików,<br />
05-870 Blonie, Poland; 3 Danko Plant Breeders Ltd., Choryń 27,64-000 Kościan, Poland; 4 Małopolska<br />
Plant Growing Company – HBP LLC, 4 Zbożowa str., 30-002 Kraków, Poland; 5 Plant Breeding<br />
Strzelce Ltd., 20 Główna str., 99-307 Strzelce, Poland; 6 Plant Breeding Smolice Ltd., Smolice 41, 63-<br />
740 Kobylin, Poland; 7 Poznań Plant Breders Ltd., 5 Kasztanowa str., 03-004 Tulce, Poland<br />
E-mail: hwis@igr.poznan.pl<br />
Fusarium head blight (FHB) is an important cereal disease caused by Fusarium<br />
species. Disease is mainly damaging for bread wheat. The aim <strong>of</strong> this work was<br />
(1) to evaluate FHB resistance variability <strong>of</strong> wheat lines and (2) to analyze<br />
concentration <strong>of</strong> Fusarium toxins in grain. This research was conducted on 71<br />
winter wheat lines differing in genetic background. Cultivars ‘Arina’ and ‘Tonacja’<br />
were used as controls. Lines were sown in two field experiments located in<br />
Cerekwica, Western Poland and in Radzików, Central Poland. Field experiments<br />
were established as a randomized complete block design. Wheat heads were<br />
inoculated at flowering stage with conidial suspension <strong>of</strong> Fusarium culmorum<br />
isolates producing deoxynivalenol (DON), nivalenol (NIV) and zearalenon (ZEA),<br />
at a rate <strong>of</strong> 100 000 spores per mL. The disease was rated 14 and 16 days after<br />
inoculation. Fusarium head blight index (FHBi) was calculated.<br />
FHBi ranged from 8.3 to 49.9%. Only 3 lines showed infection below 10%.<br />
Percentage <strong>of</strong> Fusarium damaged kernels (FDK) was high, ranging from 50.2% to<br />
98.0%. The FDKs <strong>of</strong> ‘Tonacja’ and ‘Arina’ cultivars were also high (65.2 and<br />
69.7%). However, the FDK <strong>of</strong> line ‘DED’ (susceptible check) was 82.2%.<br />
Fusarium damaged kernels <strong>of</strong> only 5 genotypes did not exceed 60.0%. The<br />
correlation coefficient <strong>of</strong> FHBi versus FDK was significant (r=0.575).<br />
Grain from 35 lines from two locations, with low FHBi and FDK, was analyzed for<br />
the presence <strong>of</strong> Fusarium toxins. DON amount was the highest, and ranged from<br />
7.041 ppm to 22.651ppm. The total amount <strong>of</strong> NIV was also high, ranging from<br />
3.331 ppm to 19.285 ppm. The lowest concentration <strong>of</strong> ZEA was found in ‘Arina’ –<br />
128 ppb, the highest ZEA level was 8432 ppb.<br />
Research was supported by the Ministry <strong>of</strong> Agriculture and Rural Development<br />
projects: HOR hn 801 - 13/11 and HOR hn 078-801-9/11.<br />
Keywords: Fusarium, mycotoxin, resistance, wheat<br />
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