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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,
VARIETY DEVELOPMENT
P94 - Resistance of winter wheat breeding lines to
Fusarium head blight and accumulation of Fusarium
toxins in grain
H. Wiśniewska 1 , T. Góral 2 , P. Ochodzki 2 , D. Walentyn-Góral 2 , J. Belter 1 , M.
Kwiatek 1 , J. Bogacki 3 , T. Drzazga 4 , B. Ługowska 3 , P. Matysik 5 , E. Witkowski 6 ,
K. Rubrycki 7 , U. Woźna–Pawlak 7
1 Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska str., 60-479 Poznan,
Poland; 2 Department of Plant Pathology, Plant Breeding and Acclimatization Institute NRI, Radzików,
05-870 Blonie, Poland; 3 Danko Plant Breeders Ltd., Choryń 27,64-000 Kościan, Poland; 4 Małopolska
Plant Growing Company – HBP LLC, 4 Zbożowa str., 30-002 Kraków, Poland; 5 Plant Breeding
Strzelce Ltd., 20 Główna str., 99-307 Strzelce, Poland; 6 Plant Breeding Smolice Ltd., Smolice 41, 63-
740 Kobylin, Poland; 7 Poznań Plant Breders Ltd., 5 Kasztanowa str., 03-004 Tulce, Poland
E-mail: hwis@igr.poznan.pl
Fusarium head blight (FHB) is an important cereal disease caused by Fusarium
species. Disease is mainly damaging for bread wheat. The aim of this work was
(1) to evaluate FHB resistance variability of wheat lines and (2) to analyze
concentration of Fusarium toxins in grain. This research was conducted on 71
winter wheat lines differing in genetic background. Cultivars ‘Arina’ and ‘Tonacja’
were used as controls. Lines were sown in two field experiments located in
Cerekwica, Western Poland and in Radzików, Central Poland. Field experiments
were established as a randomized complete block design. Wheat heads were
inoculated at flowering stage with conidial suspension of Fusarium culmorum
isolates producing deoxynivalenol (DON), nivalenol (NIV) and zearalenon (ZEA),
at a rate of 100 000 spores per mL. The disease was rated 14 and 16 days after
inoculation. Fusarium head blight index (FHBi) was calculated.
FHBi ranged from 8.3 to 49.9%. Only 3 lines showed infection below 10%.
Percentage of Fusarium damaged kernels (FDK) was high, ranging from 50.2% to
98.0%. The FDKs of ‘Tonacja’ and ‘Arina’ cultivars were also high (65.2 and
69.7%). However, the FDK of line ‘DED’ (susceptible check) was 82.2%.
Fusarium damaged kernels of only 5 genotypes did not exceed 60.0%. The
correlation coefficient of FHBi versus FDK was significant (r=0.575).
Grain from 35 lines from two locations, with low FHBi and FDK, was analyzed for
the presence of Fusarium toxins. DON amount was the highest, and ranged from
7.041 ppm to 22.651ppm. The total amount of NIV was also high, ranging from
3.331 ppm to 19.285 ppm. The lowest concentration of ZEA was found in ‘Arina’ –
128 ppb, the highest ZEA level was 8432 ppb.
Research was supported by the Ministry of Agriculture and Rural Development
projects: HOR hn 801 - 13/11 and HOR hn 078-801-9/11.
Keywords: Fusarium, mycotoxin, resistance, wheat
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