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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P90 - Chemotype-specific Fusarium isolates applied for phenotyping of type II resistance to FHB in wheat M. Ittu 1 , L. Cana 1 , P. Cornea 2 , V. Gagiu 3 1 National Agricultural Research Development Institute (NARDI) Fundulea, 1 N. Titulescu street, 915200 Romania - 2 CBAB Biotehnol, Faculty of Biotechnology of the Agronomical Science and Veterinary Medicine University , 59 Marasti Blv, Bucharest, Romania - 3 National Institute of Research&Development for Food Bioresources (IBA), 5 Ancuta Baneasa street, 020323, Bucharest, Romania E-mail: ittum@ricic.ro Fusarium head blight (FHB) is one of the most important wheat diseases throughout the world. Development and deployment of varieties resistant to FHB is the most effective option to minimize losses and is of major concern in wheat breeding program from NARDI-Fundulea. Occurrence of 3-ADON chemotype in some local Romanian Fusarium populations, in which previously the chemotype 15-ADON was prevalent as in many regions from North America and Central Europe (Ward et al, FGB, 2008, Miedaner and Talas, Int. Symp. Fusarium Head Blight, 2012), has been reported (Ittu et al, Romanian Agricultural Research, 2012). As a consequence, comparative assessment of host resistance to both chemotypes is required from the breeding perspective. The objectives of this study were to: (1)evaluate the aggressiveness of chemotype-specific 3ADON and 15ADON Fusarium isolates and (2)investigate the corresponding resistance Type II to FHB in wheat cultivars and breeding lines. 40 Romanian and international wheat genotypes were point inoculated under field condition with six selected Fusarium isolates, three of each 3-ADON and 15-ADON chemotypes (years 2011 and 2012). Very significant differences among the both Fusarium chemotypes and the chemotype-specific isolates were found, the amount of disease and pathogen spread across all wheat genotypes being in 2011 on average higher in 3-ADON isolates for all FHB traits: severity, %(34.8); AUDPC (324.6); FDK,%(67.0) as compared to 15ADON isolates: severity, %(21.0); AUDPC (190); FDK,%(44.0). The overall DON contamination of grains in both environments was higher in entries ranked relatively as susceptible (85-120ppm/kg), in comparison to the resistant ones (>25-68.4 ppm/kg), across the isolates of each Fusarium chemotype. With a few exceptions the resistance scores were better in most resistant genotypes, irrespective of Fusarium chemotype. These findings suggest the importance to develop germplasm with higher tolerance to DON contamination, if increase of divergent Fg populations become problematic and different mechanisms for resistance to pathogen and associated mycotoxins could be found. Keywords: resistance, aggressiveness, DON chemotype, wheat 183
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P91 - Mapping QTLs for Fusarium head blight response in a durum wheat elite population M. Maccaferri 1 , S. Corneti 1 , A. Ricci 1 , S. Stefanelli 1 , E. Braida 2 , P. Lancioni 2 , K. Ammar 3 , A. Prodi 1 , A. Pisi 1 , M. Pascale 4 , V. Lippolis 4 , A. Massi 2 , R. Tuberosa 1 . 1 Department of Agricultural Science - University of Bologna, Viale Fanin 44, 40127 Bologna-Italy; 2 Società Produttori Sementi Bologna, Via Macero 1, 40050 Argelato- Italy; 3 CIMMYT, Carretera Mexico-Veracruz KM. 45, 56130 Texcoco-Mexico; 4 Institute of Sciences of Food Production (ISPA), National Research Council of Italy (CNR), via G. Amendola 122/O, 70126 Bari-Italy E-mail: marco.maccaferri@unibo.it Durum wheat is strongly affected by Fusarium head blight (FHB). Thus, understanding the genetic basis of resistance is a major objective of breeding. In this study, a population of 249 F7:8 recombinant inbred lines (RILs) obtained from the cross Kofa (desertDurum®) x Svevo (Italia cultivar) has been evaluated for FHB response in five artificially inoculated field trials (mix suspension of F. graminearum and F. culmorum conidia) carried out in 2010 and 2011 in Italy and Mexico. Fusarium incidence and severity, grain yield, number of grains per spike, percentage of fusarium-damaged kernels, thousand kernel weight, deoxynivalenol (DON) content were recorded. Heritability values of FHB symptoms were medium to high across trials (from 35 to 62%). Both Kofa and Svevo showed intermediate responses, while the RILs showed extensive transgressive segregation, allowing us to map 14 QTL clusters for FHB responses and DON content. Only two of these QTL clusters, on chromosomes 2A and 7B, were coincident with QTLs for heading date. Two major QTLs consistently proved to affect FHB response across trials were located on chromosome arms 2BL and 3BS, in coincidence with major QTLs for plant height, peduncle length, senescence and grain yield previously identified in this population (Maccaferri et al. Genetics 178: 489-511). In both cases, the alleles responsible for constitutive reduced height and peduncle length, increased senescence rate and decreased grain yield were also associated to increased fusarium susceptibility. The role of these two QTLs is being further investigated with near isogenic lines, particularly to elucidate the pleiotropic vs. tight linkage relationships, in view also of the FHB escape associated to plant eight. Four QTLs for DON content were also found, two of which were DONspecific and did not influence other disease symptoms. The contribution of "AGER - Agroalimentare e Ricerca", project “From Seed to Pasta” is acknowledged. Keywords: FHB, Triticum durum, molecular markers, marker assisted selection 184
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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