EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
Create successful ePaper yourself
Turn your PDF publications into a flip-book with our unique Google optimized e-Paper software.
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P90 - Chemotype-specific Fusarium isolates applied<br />
for phenotyping <strong>of</strong> type II resistance to FHB in wheat<br />
M. Ittu 1 , L. Cana 1 , P. Cornea 2 , V. Gagiu 3<br />
1 National Agricultural Research Development Institute (NARDI) Fundulea, 1 N. Titulescu street,<br />
915200 Romania - 2 CBAB Biotehnol, Faculty <strong>of</strong> Biotechnology <strong>of</strong> the Agronomical Science and<br />
Veterinary Medicine University , 59 Marasti Blv, Bucharest, Romania - 3 National Institute <strong>of</strong><br />
Research&Development for Food Bioresources (IBA), 5 Ancuta Baneasa street, 020323, Bucharest,<br />
Romania<br />
E-mail: ittum@ricic.ro<br />
Fusarium head blight (FHB) is one <strong>of</strong> the most important wheat diseases<br />
throughout the world. Development and deployment <strong>of</strong> varieties resistant to FHB<br />
is the most effective option to minimize losses and is <strong>of</strong> major concern in wheat<br />
breeding program from NARDI-Fundulea.<br />
Occurrence <strong>of</strong> 3-ADON chemotype in some local Romanian Fusarium<br />
populations, in which previously the chemotype 15-ADON was prevalent as in<br />
many regions from North America and Central Europe (Ward et al, FGB, 2008,<br />
Miedaner and Talas, Int. Symp. Fusarium Head Blight, 2012), has been reported<br />
(Ittu et al, Romanian Agricultural Research, 2012). As a consequence,<br />
comparative assessment <strong>of</strong> host resistance to both chemotypes is required from<br />
the breeding perspective. The objectives <strong>of</strong> this study were to: (1)evaluate the<br />
aggressiveness <strong>of</strong> chemotype-specific 3ADON and 15ADON Fusarium isolates<br />
and (2)investigate the corresponding resistance Type II to FHB in wheat cultivars<br />
and breeding lines. 40 Romanian and international wheat genotypes were point<br />
inoculated under field condition with six selected Fusarium isolates, three <strong>of</strong> each<br />
3-ADON and 15-ADON chemotypes (years 2011 and 2012).<br />
Very significant differences among the both Fusarium chemotypes and the<br />
chemotype-specific isolates were found, the amount <strong>of</strong> disease and pathogen<br />
spread across all wheat genotypes being in 2011 on average higher in 3-ADON<br />
isolates for all FHB traits: severity, %(34.8); AUDPC (324.6); FDK,%(67.0) as<br />
compared to 15ADON isolates: severity, %(21.0); AUDPC (190); FDK,%(44.0).<br />
The overall DON contamination <strong>of</strong> grains in both environments was higher in<br />
entries ranked relatively as susceptible (85-120ppm/kg), in comparison to the<br />
resistant ones (>25-68.4 ppm/kg), across the isolates <strong>of</strong> each Fusarium<br />
chemotype.<br />
With a few exceptions the resistance scores were better in most resistant<br />
genotypes, irrespective <strong>of</strong> Fusarium chemotype.<br />
These findings suggest the importance to develop germplasm with higher<br />
tolerance to DON contamination, if increase <strong>of</strong> divergent Fg populations become<br />
problematic and different mechanisms for resistance to pathogen and associated<br />
mycotoxins could be found.<br />
Keywords: resistance, aggressiveness, DON chemotype, wheat<br />
183