EFS12- Book of abstracts - Contact

SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,
VARIETY DEVELOPMENT
P88 - Variation for Fusarium head blight resistance
and Fusarium toxins accumulation in winter triticale
lines inoculated with Fusarium culmorum
T. Góral 1 , H. Wiśniewska 2 , P. Ochodzki 1 , D. Walentyn-Góral 1 , I. Grzeszczak 1 ,
J. Beletr 2 , Z. Banaszak 3 , M. Pojmaj 3 , D. Kurleto 3 , M. Konieczny 3 , G.
Budzianowski 4 , A. Cicha 4 , K. Paizert 4 , H. Woś 4
1 Department of Plant Pathology, Plant Breeding and Acclimatization Institute NRI, Radzików, 05-870
Blonie, Poland; 2 Institute of Plant Genetics, Polish Academy of Sciences, 34 Strzeszyńska str., 60-479
Poznan, Poland; 3 Danko Plant Breeders Ltd., Choryń 27,64-000 Kościan, Poland; 4 Plant Breeding
Strzelce Ltd., 20 Główna str., 99-307 Strzelce, Poland
E-mail: t.goral@ihar.edu.pl
Resistance to Fusarium head blight of 29 winter triticale lines, and three cultivars
‘Borwo’, ‘Fredro’ and ‘Mikado’ was evaluated. Triticale was sown in the field
experiments in Cerekwica near Poznań and in Radzików near Warsaw. At
flowering, triticale heads were inoculated with three Fusarium culmorum isolates.
Fusarium head blight index was scored and after the harvest percentage of
Fusarium damaged kernels was assessed. Triticale grain was analyzed for the
contents of trichothecenes B (deoxynivalenol and derivatives, nivalenol) and
zearalenone.
The average FHB indexes were similar in both locations and amounted 19.8% in
Radzikow, and 19.9%. in Cerekwica. Percentage of Fusarium damaged kernels
was higher in Cerkwica (53.7%) than in Radzików (26.8%). An average content of
DON in Radzików amounted to 8,690 ppm and was lower than in the second
location – 19.543 ppm. In Cerekwica there were also large quantities of NIV in
grain. The average content was 10.048 ppm, while in Radzików it was very low –
0.324 ppm. Considerable amounts of DON derivatives in grain in both locations
were detected (1,815 ppm of 3AcDON and 1,913 ppm of 15AcDON). The content
of the ZON in the grain from Cerekwica was very high and amounted to 1123 ppb,
while in Radzików was 6 times lower - 200 ppb. Concentrations of trichotecenes B
in both locations as well as zearalenone in both locations correlated significantly.
Relationships between FHB index and mycotoxin contents were statistically
insignificant in both locations. In contrast, FDK percentages correlated
significantly with mycotoxin contents.
In both locations the parallel experiments with 36 winter wheat were carried out.
Triticale proved to be less infected than wheat (ears, kernels). However, content
of mycotoxins (trichothecenes), was higher in triticale grain than in wheat grain.
These results showed that there is a threat of contamination of triticale grain with
mycotoxins despite weaker FHB symptoms.
Keywords: Fusarium, mycotoxins, resistance, triticale
181