EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P87 - Developing test method to oats and barley for<br />
resistance to Fusarium langsethiae<br />
P. Parikka, M. Jalli<br />
MTT Agrifood Research Finland, Plant Production Research, FI-31600 Jokioinen, Finland<br />
E-mail: paivi.parikka@mtt.fi<br />
Importance <strong>of</strong> T-2/HT-2 toxins produced by F. langsethiae has increased recently<br />
in many countries, especially in Northern Europe and presence <strong>of</strong> toxins<br />
particularly in oats, raises questions <strong>of</strong> resistance in cultivars. Testing resistance<br />
in field conditions is difficult because <strong>of</strong> competing infections. F. langsethiae is the<br />
earliest Fusarium species infecting flowers and it has been detected in oats and<br />
barley at heading. Normally, Fusarium infections are dependent on humidity and<br />
infections in dry conditions are rare. F. langsethiae, however, can establish<br />
infections in field conditions without long-lasting high relative humidity.<br />
Development <strong>of</strong> greenhouse test to F. langsethiae was started at MTT Agrifood<br />
Research Finland in 2009 first on oats and later also some barley cultivars were<br />
included in the test calibration. Development and transmission <strong>of</strong> infection was<br />
studied by inoculating seedlings and developing plants at different growth stages:<br />
starting at young seedling stage and ending at panicle emergence and flowering.<br />
Inoculations with F. langsethiae spore suspension were made with a hand<br />
sprayer. Temperature and humidity were regulated according to plant<br />
development and inoculation periods. High humidity was maintained in<br />
greenhouse shortly before and after inoculation. Observations <strong>of</strong> infection were<br />
made one and two weeks after inoculation and <strong>of</strong> harvested grain by plating<br />
kernels on peptone-PCNB agar plates.<br />
F. langsethiae sprayed on young oat seedlings did not continue development<br />
further in plants and did not infect panicles. Inoculation at shoot development was<br />
also unable to proceed up to panicles, and when sprayed at flag leaf stage the<br />
fungus could only occasionally infect panicles. Successful infections were<br />
obtained when plants were inoculated at head/panicle emergence or one week<br />
after that. In oats, the later inoculations resulted in higher infections. Differences<br />
between cultivars could be obtained in observations both in oats and barley.<br />
Keywords: cultivars, resistance, infection<br />
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