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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P86 - Selection of aggressive Fusarium isolates for breeding M. Ittu 1 , I. Ciocazanu 2 1 National Agricultural Research Development Institute (NARDI) Fundulea, 1 N. Titulescu street, 915200 Romania; 2 Pioneer Hi-Breed Seed Agro SRL, com. Ganeasa, sat Sindrilita DN 2, km 19,7, jud. Ilfov, 077010, Romania E-mail: ittum@ricic.ro Fusarium species from Gibberella fujikuroi complex (F.verticillioides, F.proliferatum and/or F. subglutinans) are important fungal pathogens that cause pink ear rot (PER), a disease of maize with devastating impact on yield losses and food safety. Use of reliable Fusarium isolates for assessment of host resistance and understanding of relation between phenotypic scoring and Fumonisin (FUM) accumulation are crucial for developing strategies to minimize the associated risks to disease. The objectives of this study were to evaluate: i) selection of aggressive isolates for large scale inoculums production in several Fusarium populations, sampled from South Romania and ii) relation between disease scores and FUM content under artificial field inoculation. New pathogenic isolates are obtained each two years, by specific procedures. Aggressiveness of the new isolates was evaluated under artificial inoculation in seedling stage, data being expressed as reduction of coleoptyle length in inoculated seedlings vs. noninoculated ones (as % of control). Isolates causing greatest reduction in coleoptiles growth were considered as most aggressive and were selected for large scale production of inoculum for breeding purposes. Multienvironment assessment of resistance under artificial field inoculations performed with highly aggressive F. verticillioides isolates, obtained in this way, revealed a very close negative correlation between pink ear rot rating (1=very sensitive, 9=very resistant) and FUM content (ppm) across years, according to correlation index that ranged from r=-0.729, N=61 (2008) to r=-0,815, N=15 (2010). Based on these findings it could be assumed that selection for reduced symptoms (pink ear rot score) should fairly allow identification of genotypes with reduced FUM content. Keywords: aggressiveness, Fumonisin (FUM), resistance, maize 179
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P87 - Developing test method to oats and barley for resistance to Fusarium langsethiae P. Parikka, M. Jalli MTT Agrifood Research Finland, Plant Production Research, FI-31600 Jokioinen, Finland E-mail: paivi.parikka@mtt.fi Importance of T-2/HT-2 toxins produced by F. langsethiae has increased recently in many countries, especially in Northern Europe and presence of toxins particularly in oats, raises questions of resistance in cultivars. Testing resistance in field conditions is difficult because of competing infections. F. langsethiae is the earliest Fusarium species infecting flowers and it has been detected in oats and barley at heading. Normally, Fusarium infections are dependent on humidity and infections in dry conditions are rare. F. langsethiae, however, can establish infections in field conditions without long-lasting high relative humidity. Development of greenhouse test to F. langsethiae was started at MTT Agrifood Research Finland in 2009 first on oats and later also some barley cultivars were included in the test calibration. Development and transmission of infection was studied by inoculating seedlings and developing plants at different growth stages: starting at young seedling stage and ending at panicle emergence and flowering. Inoculations with F. langsethiae spore suspension were made with a hand sprayer. Temperature and humidity were regulated according to plant development and inoculation periods. High humidity was maintained in greenhouse shortly before and after inoculation. Observations of infection were made one and two weeks after inoculation and of harvested grain by plating kernels on peptone-PCNB agar plates. F. langsethiae sprayed on young oat seedlings did not continue development further in plants and did not infect panicles. Inoculation at shoot development was also unable to proceed up to panicles, and when sprayed at flag leaf stage the fungus could only occasionally infect panicles. Successful infections were obtained when plants were inoculated at head/panicle emergence or one week after that. In oats, the later inoculations resulted in higher infections. Differences between cultivars could be obtained in observations both in oats and barley. Keywords: cultivars, resistance, infection 180
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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