EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM,<br />
VARIETY DEVELOPMENT<br />
P83 - Advantage <strong>of</strong> using native sources <strong>of</strong> FHB<br />
resistance in breeding winter wheat in Ontario,<br />
Canada<br />
L. Tamburic-Ilincic<br />
University <strong>of</strong> Guelph, Ridgetown Campus, 120 Main St.E. Ridgetown, Ontario, N0P2C0, Canada<br />
E-mail: ltamburi@uoguelph.ca<br />
Fusarium head blight (FHB) is one <strong>of</strong> the most serious diseases <strong>of</strong> wheat. FHB<br />
reduces grain yield and quality, and the fungus produces mycotoxins, such as<br />
deoxynivalenol (DON). The most practical way to control FHB is through the<br />
development <strong>of</strong> resistant cultivars. In addition to exotic sources <strong>of</strong> FHB resistance<br />
(such as ‘Sumai 3’ and ‘Frontana’), ‘native’ sources <strong>of</strong> resistance are commonly<br />
used in wheat breeding programs in North America. ‘Native’ winter wheat has<br />
better quality and higher yield compared to ‘exotic’ wheat and progenies with<br />
good quality, yield and unique FHB resistance could be identified faster. In the<br />
current study, we developed a double haploid (DH) population from cross Pioneer<br />
25R47 (susceptible to FHB) and Vienna (moderately resistant to FHB). 110 DH<br />
lines were screened for FHB resistance in 2010, 2011 and 2012. Each line was<br />
inoculated with a combined suspension <strong>of</strong> macroconidia <strong>of</strong> four Fusarium<br />
graminearum isolates at 50% anthesis. Plots were misted daily beginning after the<br />
first plots were inoculated until three days after the last plots were inoculated.<br />
FHB symptoms were recorded as severity (the percent spikelets infected) and<br />
incidence (the percent heads infected) and a fusarium head blight index (FHBI)<br />
was calculated as the product <strong>of</strong> severity and incidence divided by 100. The<br />
harvested grain was analyzed for DON level using ELISA method (EZ-Quant®<br />
www.diagnostix.ca). Average FHBI, DON level and yield for Pioneer 25R47 was<br />
14%, 6.3 ppm and 4.9 t/ha and for Vienna was 4.9%, 4.6 ppm and 5.0 t/ha.<br />
Several breeding lines with lower FHB index and DON level than Vienna were<br />
identified. The highest yield across the population was 5.4 t/ha. These results<br />
indicate that progenies, with better performance than parents, should be identified<br />
in relatively short period <strong>of</strong> time by using DH technology and ‘native’ sources <strong>of</strong><br />
FHB resistance.<br />
Keyword: Fusarium, wheat, resistance<br />
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