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SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P82 - Interaction between Quantitative Trait Loci (QTL) for Fusarium head blight (FHB) resistance and Fusarium graminearum 15-ADON and 3-ADON chemotypes in spring wheat L. Tamburic-Ilincic, A. Muckle, A. Schaafsma University of Guelph, Ridgetown Campus, 120 Main St. E. Ridgetown, Ontario, N0P2C0, Canada E-mail: ltamburi@uoguelph.ca Fusarium graminearum (Schwabe) (FG) is the principal cause of Fusarium head blight (FHB) in North America, one of the most serious diseases of wheat (Triticum aestivum L.). Deoxynivalenol (DON) is the most important mycotoxin produced by FG. 15-acetyl DON (15-ADON) and 3-acetyl DON (3-ADON) chemotypes of FG produce DON and 15-ADON and DON and 3-ADON analogs, respectively. Quantitative trait loci (QTL) associated with FHB resistance from a Wuhan x Nyubai spring wheat population were previously published. In the present study we investigated: 1) which QTL or QTL combination (no QTL, 3B, 5A and 3B+5A) result in the lowest FHB symptoms and DON accumulation and 2) whether FG chemotype and QTL class interacted. We used eight lines (two from each QTL class) and six FG isolates (three from each chemotype group) in field experiments conducted over three years. Wheat lines were spray-inoculated at 50% anthesis with FG isolates and water (control). FHB symptoms were recorded as severity and incidence and their product calculated as FHB index. The harvested grain was analyzed for total DON using ELISA method, while 15-ADON and 3-ADON analogs were confirmed using gas chromatography-mass spectrometry (GC-MS). The lowest level of total DON and FHB index was recorded in wheat lines from the 3B QTL class. In fact lines carrying both QTLs had higher DON content than lines carrying each QTL individually. Lines inoculated with either 15-ADON or 3-ADON FG reacted similarly, with no interaction between chemotype and QTL class. Our results suggest that breeding for FHB resistance in this population using only the 3B QTL is the best strategy for development of wheat lines with lower DON level and FHB index regardless of the FG chemotype used in the inoculations. Keywords: Fusarium, wheat, resistance, mycotoxins 175
SESSION 4: GENETICS OF HOSTS – PLANT RESISTANCE TO FUSARIUM, VARIETY DEVELOPMENT P83 - Advantage of using native sources of FHB resistance in breeding winter wheat in Ontario, Canada L. Tamburic-Ilincic University of Guelph, Ridgetown Campus, 120 Main St.E. Ridgetown, Ontario, N0P2C0, Canada E-mail: ltamburi@uoguelph.ca Fusarium head blight (FHB) is one of the most serious diseases of wheat. FHB reduces grain yield and quality, and the fungus produces mycotoxins, such as deoxynivalenol (DON). The most practical way to control FHB is through the development of resistant cultivars. In addition to exotic sources of FHB resistance (such as ‘Sumai 3’ and ‘Frontana’), ‘native’ sources of resistance are commonly used in wheat breeding programs in North America. ‘Native’ winter wheat has better quality and higher yield compared to ‘exotic’ wheat and progenies with good quality, yield and unique FHB resistance could be identified faster. In the current study, we developed a double haploid (DH) population from cross Pioneer 25R47 (susceptible to FHB) and Vienna (moderately resistant to FHB). 110 DH lines were screened for FHB resistance in 2010, 2011 and 2012. Each line was inoculated with a combined suspension of macroconidia of four Fusarium graminearum isolates at 50% anthesis. Plots were misted daily beginning after the first plots were inoculated until three days after the last plots were inoculated. FHB symptoms were recorded as severity (the percent spikelets infected) and incidence (the percent heads infected) and a fusarium head blight index (FHBI) was calculated as the product of severity and incidence divided by 100. The harvested grain was analyzed for DON level using ELISA method (EZ-Quant® www.diagnostix.ca). Average FHBI, DON level and yield for Pioneer 25R47 was 14%, 6.3 ppm and 4.9 t/ha and for Vienna was 4.9%, 4.6 ppm and 5.0 t/ha. Several breeding lines with lower FHB index and DON level than Vienna were identified. The highest yield across the population was 5.4 t/ha. These results indicate that progenies, with better performance than parents, should be identified in relatively short period of time by using DH technology and ‘native’ sources of FHB resistance. Keyword: Fusarium, wheat, resistance 176
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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