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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P78 - Molecular characterization of Fusarium species occurring on olive fruits in Apulia S. Frisullo 1 , A. Susca 2 , G. Stea 2 , A. Villani 2 , L. Prudente 1 , M. Contursi 1 , P. Ferrara 1 , A. Logrieco 2 , A. Moretti 2 1 Università di degli Studi di Foggia, Dipartimento di Scienze Agrarie, degli Alimenti e dell’Ambiente, Foggia, Italy; 2 Institute of Sciences of Food Production, CNR, Bari, Italy E-mail: antonio.moretti@ispa.cnr.it Olive cultivation is one of the most important crop in Apulia, with 377,526 hectares cultivated and 10,102,300 quintals of olive production. In a survey aimed to evaluate the fungal colonization of olive fruits carried out in the whole Apulia Region, 5 fields for each of the 92 localities selected were investigated. From the survey, together with fungal strains belonging to Botriosphaeriae, Colletotrichum, Diplodia, Neofusicoccum and Penicillium genera, several hundreds of strains belonging to Fusarium genus have been isolated mainly from olive fruits and, at a lesser extent, also from branches. The strains of Fusarium were identified at a morphologically level, resulting species able to produce a wide range of mycotoxins such as cyclohesadepsipeptides, moniliformin and trichothecenes. However, since each Fusarium species can have a specific mycotoxin profile, the toxicological risk related to their occurrence can be highly variable, according with the main species colonizing the olive fruits and must be accurately assessed. Confirmation of strain identification was carried out by using molecular approach. One-hundred and forty-eight representative strains were analyzed by sequencing a portion of calmodulin and β-tubulin genes, which have been proved to be effective for distinguishing species in Fusarium. Data have shown, for the first time, a wide genetic diversity within the population of Fusarium isolated from olives. In particular, F. acuminatum, F. avenaceum, F. longipes, F. merismoides, F. oxysporum, F. proliferatum, F. solani, and F. torulosum were identified. However, many strains could not be assigned to any species and therefore may represent new entities within the genus. The occurrence of some highly toxigenic Fusarium species suggests that a toxicological risk can occur in olive fruits highly contaminated by Fusarium and that such risk must be constantly monitored, also in order to evaluate possible influence of climatic changes on the Fusarium spread on this crop in Apulia. Keywords: olive fruits, Fusarium acuminatum, calmodulin, β-tubulin 171
SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P79 - Evaluation of Fusarium wilt resistance among the accessions of eggplant (Solanum melongena L.) H. H. Altinok 1 , C. Can 2 , F. Boyaci 3 , V. Topçu 3 1 Department of Plant Protection, Faculty of Agriculture, Erciyes University, Kayseri, Turkey; 2 Department of Biology, Faculty of Science, Gaziantep University, Gaziantep, Turkey; 3 West Mediterranean Agricultural Research Institute, Antalya, Turkey E-mail: ahandan@gmail.com Fusarium wilt (Fusarium oxysporum (Schlechtend: Fr.) f. sp. melongenae; Fomg) is one of the most destructive and widely distributed diseases of eggplant in Turkey. Resistant cultivars are yet to be developed against this pathogen. In this study, eggplant accessions were tested with three highly virulent Fomg isolates (MS-7, UBR-9 and Fomg10) in order to evaluate the presence of field resistance to Fusarium wilt. The plant material for the resistance screening study consisted of 13 accessions of eggplant (Solanum melongena L.), namely, “AGR-703, Amadeo F1, Anatolia F1, Angela F1, Brigitte F1 Corsica F1, Hadrian, Hawk, Kemer, Köksal, Nouma, Sharapova, Yula”. Observations were recorded from leaves at 7 th , 11 th , 14 th , 18 th 21 st , and 25 th days using a disease rating scale. The disease severity (%) and the area under disease progress curve (AUDPC) for each of the Fomg isolates were calculated by scale values. Disease incidence ranged from 10% to 95% at eggplant seedlings. According to test results, AGR-703 was found resistant; Köksal and Hawk were moderately resistant, Amadeo F1, Corsica F1, Anatolia F1, Sharapova, Brigitte F1, Angela F1, Nouma and Yula were moderately susceptible and Hadrian and Kemer were identified as susceptible. Due to its strong resistance against the pathogen, among the all accessions tested, AGR- 703 is considered as the most appropriate for rootstock in eggplant cultivation where Fusarium wilt is present in the soil. Keywords: Fusarium wilt, cultivar, rootstock, disease severity, eggplant 172
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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