EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION<br />
GENETICS<br />
P74 - The molecular characterization and<br />
determination <strong>of</strong> genetic variability in Fusarium<br />
verticilloides strains isolated from maize in Turkey<br />
B. Kansu 1 , P. Marin 2 , M. T. Gonzalez-Jaen 2 , B. Tunali 1<br />
1 Ondokuz Mayis University, Agriculture Faculty, Department <strong>of</strong> Plant Protection, 55139, Samsun-<br />
Turkey; 2 Department <strong>of</strong> Genetics, Faculty <strong>of</strong> Biology, Complutense University <strong>of</strong> Madrid, Jose Antonio<br />
Novais 12, 28040 Madrid-Spain<br />
E-mail: kansu_bayram@yahoo.com<br />
Maize ear rot is one <strong>of</strong> the most important and common disease in maize fields <strong>of</strong><br />
Turkey, as well as all over the world. The causal agents <strong>of</strong> this disease include<br />
some <strong>of</strong> the Fusarium species, but Fusarium verticilloides (Gibberella<br />
moniliformis, G. fujikuroi mating population A) is considered the main responsible<br />
for decreasing yield in fields. Additionally this species produces several<br />
mycotoxins, especially fumonisins, which are dangerous for human and livestock.<br />
In order to devise effective strategies to reduce both pathogen growth and<br />
fumonisin risk it is necessary to gain knowledge about the genetic variability and<br />
the population structure to detect potential populations and lineages that might<br />
exist in terms <strong>of</strong> toxin pr<strong>of</strong>ile, response to antifungal agents or differential climatic<br />
features. These aspects were studied using the partial sequences <strong>of</strong> the IGS<br />
region (Intergenic Spacer <strong>of</strong> rDNA units) and the translation elongation factor 1 α<br />
gene (EF1-α) <strong>of</strong> a representative sample (66 strains) <strong>of</strong> F. verticilloides isolated<br />
from six different agro-climatic regions <strong>of</strong> Turkey. Additional strains from diverse<br />
geographic origins were also included in the phylogenetic analyses performed<br />
using PAUP. The fumonisin-producing ability <strong>of</strong> the F. verticillioides strains was<br />
tested using a specific PCR assay based on FUM1 gene, a key gene <strong>of</strong> fumonisin<br />
biosynthetic pathway. All the strains were positive to the analysis. Additionally,<br />
mating type alleles, MAT-1 and MAT-2, were determined in all the strains.<br />
Percentages <strong>of</strong> MAT-1 and MAT-2 idiomorphs were 63.2% and 36.8%,<br />
respectively. The separate and combined IGS and EF1-α phylogenies were<br />
obtained and discussed in comparison with previous analyses reported and in<br />
relation with climatic factors.<br />
This research was supported by the Project Office <strong>of</strong> Ondokuz Mayıs University <strong>of</strong><br />
Turkey (PYO.ZRT.1904.012.010) and was supported partially by the Ministry <strong>of</strong><br />
Science and Innovation <strong>of</strong> Spain (AGL2010-22182-C04-01).<br />
Keywords: Fusarium verticilloides, IGS, EF–1, species-specific PCR<br />
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