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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P70 - ToxiFusaDB: the online catalogue of the MycSA Fusarium strains collection V. F. Wong Jun Tai 1,2 , L. Pinson-Gadais 2 , C. Fernandez 1 , C. Barreau 2 F. Richard-Forget 2 1 INRA - UMR0927 I2M, Département Génie Civil et Environnemental de l'Institut de Mécanique et d'Ingénierie de Bordeaux, 16 Avenue Pey-Berland 33607 Pessac Cedex; 2 INRA – UR1264 - Mycologie et Sécurité des Aliments (MycSA), 71 avenue Edouard Bourlaux, CS 20032, F-33882 Villenave d’Ornon Cedex E-mail: lpinson@bordeaux.inra.fr More than 100 species belong to the Fusarium genus. Among them, phytopathogenic fungi can infect various crops worldwide causing significant economic losses. In addition, toxigenic Fusaria can produce a wide array of mycotoxins and represent a serious threat for food and feed safety. Tracking and understanding Fusarium diversity is therefore critical for the development of efficient strategies to control fungal attacks and mycotoxin contamination. The MycSA research group collected more than 400 strains of Fusarium spp. [F.graminearum, F.culmorum, F.verticillioides and F.proliferatum] contaminating french wheat and corn harvests. All stored strains are genotyped using species- and chemotype-specific markers. The F. graminearum strains were further characterized with a set of 20 microsatellites markers (see poster “The French Fusarium Collection: a living resource for mycotoxin research”). The toxigenic potential - amount of toxins a strain can produce when cultivated on kernels in optimal conditions – has been determined for all Fusarium strains included in the MycSA collection. According to these original and highly interesting phenotyping data, the MycSA collection is unique in Europe. To promote communication, education, exchange and dissemination of these Fusarium characterized strains among the national and international research community, an online public access catalog has been developed. Catalogue consultation does not require specific knowledge on the Fusarium strains complexity and diversity. The catalogue can be browsed or searched using multiple criteria (Fusarium species, chemotype, toxigenic potential…), and strains of interest can be ordered online. Keywords: Fusarium, Catalogue, Distribution, Php, MySQL, HTML / CSS / JavaScript 163
SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P71 - Real time PCR for FHB quantification: bias analysis? S. Elbelt 1 , S. Fromentin 1 , V. Laval 1 , C. Barreau 2 , F. Carpentier 1 , C. Ducos 2 , C. Lannou 1 , F. Techini 1 , J. Toussaint 3 , C. Vitry 3 , L. Pinson-Gadais 2 1 INRA, UR1290 Bioger_CPP, bat 13, avenue L. Bretignière, F-78850 Thiverval Grignon, France; 2 INRA, UR1264 MycSA, 71 Avenue Edouard Bourlaux, CS 20032, F-33882 Villenave d’Ornon, France; 3ARVALIS Institut du Végétal, bat 13, avenue L. Bretignière, F-78850 Thiverval Grignon, France E-mail: vlaval@versailles.inra.fr A better understanding of the FHB species complex and particularly of the way species interactions influence epidemic development and toxin production requires accurate, sensitive and specific molecular diagnostic tools, such as realtime PCR. Nine qPCR tests specifically designed for the most represented species of the FHB complex on wheat in Europe: F. graminearum, F. culmorum, F. poae, F. avenaceum, F. langsethiae, F. tricinctum, F. sporotrichioides, M. majus and M. nivale were assessed for their potential for diagnosis. We present here the results of quantification analysis performed in three laboratories which use different mix and real time PCR apparatus. The variation in quantification will be discussed. In one laboratory the limits of the diagnostic was further studied by analysing DNA extraction and real time PCR repeatability. Statistical analysis was performed on results obtained from two extractions of 222 samples and three real time PCR analysis performed per extraction. First results show the importance to carefully test real time PCR parameters when developing real time PCR tools for diagnosis or epidemiological studies. Keywords: Molecular diagnostic tools, real-time PCR, FHB, inter-laboratory validation tests 164
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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