EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION<br />
GENETICS<br />
P67 - Genetic and mycotoxigenic diversity <strong>of</strong> isolates<br />
belonging to the Fusarium incarnatum-equiseti<br />
species complex, and recovered from maize and<br />
banana in China<br />
F. Munaut 1 , J. Scauflaire 2 , M. Gourgue 2 , C. Bivort 2 , Y. Qi 3 , A. Wu 4 , S. de<br />
Saeger 5 , D. Zhang 4 , F. Van Hove 1<br />
1 Université catholique de Louvain, Earth and Life Institute (ELI), Applied Microbiology, Mycology,<br />
Mycothèque de l’UCL (BCCM TM /MUCL), Louvain-la-Neuve, Belgium; 2 Université catholique de<br />
Louvain, Earth and Life Institute (ELI), Applied Microbiology, Mycology, Louvain-la-Neuve, Belgium;<br />
3 Academy <strong>of</strong> Tropical Agricultural Sciences (CATAS), Environment and Plant Protection Institute,<br />
Haikou, Hainan Province, China; 4 College <strong>of</strong> Life Science and Biotechnology, Shanghai Jiao Tong<br />
University (SJTU), Shangai, China; 5Universiteit Gent (UGent), Faculteit Farmaceutische<br />
Wetenschappen, Laboratory <strong>of</strong> Food Analysis, Ghent, Belgium<br />
E-mail: francoise.munaut@uclouvain.be<br />
In the frame <strong>of</strong> a research project aiming to describe the Fusarium spp diversity on maize<br />
and banana in four Chinese provinces, 80 out <strong>of</strong> the 201 isolates obtained were identified<br />
as belonging to the Fusarium incarnatum-equiseti species complex. Isolates were identified<br />
on basis <strong>of</strong> the Elongation Factor-1 a sequences (EF), which were then submitted to a<br />
phylogenetic analysis together with four isolates representative <strong>of</strong> four phylogenetic<br />
species inside the complex (F. incarnatum s.s., F. lacertarum, F. scirpi and F. equiseti). On<br />
maize, five main phylogenetic groups were determined (I, II, III, IV and V), from which three<br />
groups (I, II and V) were also recovered from banana. Surprisingly, groups I, II, III and V<br />
can be correlated to phylogenetic species described in completely different ecosystems by<br />
O’Donnell et al. (2009) (from human beings) and by Funnell-Harris et al. (2010) (from<br />
sorghum). Furthermore, one possible new phylogenetic species (group IV) was detected.<br />
Genetic diversity <strong>of</strong> the FIESC isolates was analyzed using the AFLP technique. A total <strong>of</strong><br />
200 polymorphic bands were generated for 28 FIESC isolates from maize and were used<br />
to construct a phenetic tree. The EF phylogenetic groups were clearly clustered separately<br />
by AFLP, with bootstrap values >70%. The groups II and V presented an approximate<br />
similarity <strong>of</strong> only 50% and 40 % from the other cluster, respectively. This raises the<br />
taxonomical status <strong>of</strong> the groups. Within each cluster, an important genetic diversity is<br />
observed, although no strict correlation can be made with any parameters <strong>of</strong> collect such<br />
as location, field, etc. Preliminary results on banana isolates showed similar results as well<br />
as the occurrence <strong>of</strong> an important genetic diversity.<br />
Molecular studies have demonstrated that the FIESC isolates do not possess the FUM<br />
gene cluster. Nevertheless, it has been reported that FIESC isolates are able to produce<br />
type A and B trichothecenes. Due to the fact that trichothecenes are also produced by<br />
Fusarium graminearum, which was frequently isolated from Chinese maize, the origin <strong>of</strong><br />
trichothecene contaminations on that crop has to be reconsidered. Furthermore, the<br />
occurrence <strong>of</strong> FIESC isolates on both maize and banana, which are <strong>of</strong>ten cultivated close<br />
to each other, raises the question <strong>of</strong> possible cross-contaminations.<br />
Keywords: Fusarium, FIESC, maize, banana, China, phylogeny, AFLP, mycotoxins<br />
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