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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P54 - Fusarium Head Blight agents and mycotoxin contamination in barley kernels in Italy M. Giannini 1 , G. Beccari 2 , M. Montanari 3 , L. Ricci Vitiani 2 , L. Covarelli 2 , A. Prodi 1 1 Dipartimento di Scienze Agrarie (DIPSA), Università degli Studi di Bologna, Viale Fanin 46, 40127, Bologna, Italy, 2 Dipartimento di Scienze Agrarie e Ambientali, Università degli Studi di Perugia, Borgo XX Giugno 74, 06121, Perugia, Italy, 3 INRAN Settore Sementiero, Sezione di Bologna, Via Sicilia 2, 40024 Osteria Grande, Bologna, Italy E-mail: marta.giannini2@unibo.it In 2011 in Italy about 8% of the whole surface planted with cereals was cultivated with barley, mainly intended, in order of importance, for livestock alimentation, malt industry and human food. Fusarium Head Blight (FHB) is a very important disease of wheat and barley worldwide which causes grain yield and quality reductions and that may lead to the accumulation of mycotoxins, secondary fungal metabolites that pose a serious health risk to humans and animals. The fungal species frequently associated with FHB of cereals in Europe are F. graminearum, F. culmorum and F. avenaceum, but in the last years particular importance has been given to Fusarium species belonging to the Sporotrichiella section such as F. poae and F. langsethiae, considered potential type A and B trichotechene producers.Thirty-five kernel samples of distic and polistic barley, hulless and covered, collected in Italy during 2011 in the Italian regions Emilia Romagna, Marche, Umbria and Lazio were analyzed for the presence of fungal species and for mycotoxin contamination. Pure cultures of Fusarium spp. were identified both morphologically by microscopy and molecularly by species-specific PCR assays. Furthermore, ELISA assays were performed on milled kernels for deoxynivalenol (DON) and T-2 toxin quantification. F. poae and F. tricinctum were the most detected species, followed by F. langsethiae, F. avenaceum, F. graminearum and F. equiseti. The high incidence of the first two species may justify the presence of T-2 toxin found in the grain. DON contamination was almost absent. Keywords: Fusarium, FHB, barley, T-2 toxin, deoxynivalenol 147
SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION GENETICS P55 - Occurrence of Fusarium species isolated from Barley and Bread wheat grain and detection of Deoxynivalenol in Northern Tunisia L. Gargouri Kammoun 1,2 , M. Mnari-Hattab 1 , M. Ghanmi 1 , M. Rabeh Hajlaoui 1 1 Laboratory of biotechnology applied to agriculture, the National Institute for Agricultural Research, INRA Tunisia, Rue Hedi Karray, 2049 Ariana, University of Carthage Tunisia; 2 Higher school of Agriculture of Kef, University of Jendouba, Tunisia E-mail: lobna_kammoun@yahoo.fr Cereals contaminated by Fusarium sp. and their mycotoxins, e.g. deoxynivalenol (DON) cause a variety of toxic effects on humans and animal health throughout the world. This study examines the occurrence and the influence of Fusarium infection levels of barley and bread wheat grain on its quality. This study was carried in Northern Tunisia in 2011 and 2012 crop year. Five regions including Beja, Jendouba, Bizerte, Zaghouan, and Tunis were prospected. Twenty fields were visited and morphological identification showed that 85% were identified as Fusarium culmorum, 10% as F. pseudograminearum, and 5% as F. avenaceum from Barley. However, only 30% were identified as F. culmorum from bread wheat. The amounts of trichothecene deoxynivalenol (DON) in harvested grains collected from these fields were determined by RIDASCREEN DON Enzyme Immunoassay kit (ELISA). Results indicated that the level of DON contamination in the positive samples was low, with a range of 0.2-1 ppm (mean 0.256 ppm) in 60% of barley samples, and range of 0.5-1 ppm (mean 0.3 ppm) in 30% of bread wheat samples. This is the first study with indicate the occurrence of Fusarium species on barley and bred wheat. Through our analysis we confirm the increasing importance of F. culmorum on cereals in Tunisia. Keywords: Fusarium, Barley, bread, wheat, DON 148
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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KEYNOTE LECTURE SESSION 2: SECONDAR
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SESSION 2: SECONDARY METABOLITES -
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SESSION 3: PATHOGENESIS - EPIDEMIOL
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KEYNOTE LECTURE 1 SESSION 4: GENETI
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SESSION 4: GENETICS OF HOSTS - PLAN
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KEYNOTE LECTURE 2 SESSION 4: GENETI
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KEYNOTE LECTURE SESSION 5: DISEASE
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SESSION 5: DISEASE CONTROL AND FORE
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SESSION 6: FUTURE CHALLENGE FOR EUR
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POSTER PRESENTATIONS 91
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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