EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION<br />
GENETICS<br />
P49 - Pathogenicity <strong>of</strong> Fusarium temperatum and<br />
Fusarium subglutinans on maize stalk and ear under<br />
artificial inoculation under field conditions<br />
J. Lević 1 , F. Munaut 2 , J. Scauflaire 2 , S. Stanković 1 , D. Ivanović 1 , V. Krnjaja 3<br />
1 Maize Research Institute, Zemun Polje, Belgrade-Zemun, S. Bajića 1, 11185 Belgrade, Republic <strong>of</strong><br />
Serbia; 2 Université catholique de Louvain, Earth and Life Institute, Laboratory <strong>of</strong> Mycology,<br />
Mycothèque de l’Université catholique de Louvain, Université catholique de Louvain, Croix du Sud 2,<br />
L7.05.06, 1348 Louvain-la-Neuve, Belgium; 3 Institute for Animal Husbandry, Autoput 16, 11080,<br />
Belgrade-Zemun, Republic <strong>of</strong> Serbia<br />
E-mail: jlevic@mrizp.rs<br />
Pathogenicity <strong>of</strong> Fusarium temperatum, a new species morphologically similar<br />
and phylogenetically closely related to F. subglutinans, was observed on stalk and<br />
ear <strong>of</strong> maize (Zea mays L.). In 2010, this species was established in the culture<br />
collection <strong>of</strong> the Maize Research Institute, stored since 2004. Based on<br />
interspecies mating compatibility analyses and confirmed with AFLP fingerprint<br />
pr<strong>of</strong>iles (done by Munaut), two <strong>of</strong> 20 tested F. subglutinans isolates belonged to<br />
the species F. temperatum. These isolates originated from sorghum (Sorghum<br />
bicolour (L.) Moench.) grain. In 2011 and 2012, pathogenicity <strong>of</strong> 20 (2 F.<br />
temperatum and 18 F. subglutinans, respectively) and 4 isolates (2 <strong>of</strong> each F.<br />
temperatum F. and F. subglutinans), respectively, was tested on stalk and ear <strong>of</strong><br />
two maize hybrids. The artificial inoculation <strong>of</strong> stalk was done by insertion <strong>of</strong> a<br />
Fusarium-inoculated toothpick into the second internode 7 days after silking.<br />
Control plants were inoculated with a sterile toothpick. After 6 weeks, disease<br />
intensity was evaluated on the stalk longitudinal section by the 0-6 scale (0 -<br />
necrosis localised at the inoculation spot; 6 - necrosis spreads to other<br />
internodes). Ear inoculation was done with 2-ml spore suspension in silk channels<br />
3-5 days after silking. Control plants were treated with 2 ml sterile water. The<br />
disease intensity was assessed on ears immediately prior to harvest, according to<br />
the 1-7 scale (1 - no symptoms; 7 - 76-100% infected kernels). There were not<br />
statistically significant differences in pathogenicity between isolates <strong>of</strong> F.<br />
temperatum and F. subglutinans, either on stalk or ear. Differences in hybrids<br />
responses and symptoms that isolates <strong>of</strong> fungal species caused on stalks and<br />
ears were poorly visible. According to our knowledge, the F. temperaturm<br />
occurrence on sorghum seed as well as its pathogenicity on maize ear and stalk<br />
are detected for the first time in the world.<br />
Keywords: F. temperatum, F. subglutinans, maize, sorghum<br />
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