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SESSION 3: PATHOGENESIS – EPIDEMIOLOGY AND POPULATION
GENETICS
P49 - Pathogenicity of Fusarium temperatum and
Fusarium subglutinans on maize stalk and ear under
artificial inoculation under field conditions
J. Lević 1 , F. Munaut 2 , J. Scauflaire 2 , S. Stanković 1 , D. Ivanović 1 , V. Krnjaja 3
1 Maize Research Institute, Zemun Polje, Belgrade-Zemun, S. Bajića 1, 11185 Belgrade, Republic of
Serbia; 2 Université catholique de Louvain, Earth and Life Institute, Laboratory of Mycology,
Mycothèque de l’Université catholique de Louvain, Université catholique de Louvain, Croix du Sud 2,
L7.05.06, 1348 Louvain-la-Neuve, Belgium; 3 Institute for Animal Husbandry, Autoput 16, 11080,
Belgrade-Zemun, Republic of Serbia
E-mail: jlevic@mrizp.rs
Pathogenicity of Fusarium temperatum, a new species morphologically similar
and phylogenetically closely related to F. subglutinans, was observed on stalk and
ear of maize (Zea mays L.). In 2010, this species was established in the culture
collection of the Maize Research Institute, stored since 2004. Based on
interspecies mating compatibility analyses and confirmed with AFLP fingerprint
profiles (done by Munaut), two of 20 tested F. subglutinans isolates belonged to
the species F. temperatum. These isolates originated from sorghum (Sorghum
bicolour (L.) Moench.) grain. In 2011 and 2012, pathogenicity of 20 (2 F.
temperatum and 18 F. subglutinans, respectively) and 4 isolates (2 of each F.
temperatum F. and F. subglutinans), respectively, was tested on stalk and ear of
two maize hybrids. The artificial inoculation of stalk was done by insertion of a
Fusarium-inoculated toothpick into the second internode 7 days after silking.
Control plants were inoculated with a sterile toothpick. After 6 weeks, disease
intensity was evaluated on the stalk longitudinal section by the 0-6 scale (0 -
necrosis localised at the inoculation spot; 6 - necrosis spreads to other
internodes). Ear inoculation was done with 2-ml spore suspension in silk channels
3-5 days after silking. Control plants were treated with 2 ml sterile water. The
disease intensity was assessed on ears immediately prior to harvest, according to
the 1-7 scale (1 - no symptoms; 7 - 76-100% infected kernels). There were not
statistically significant differences in pathogenicity between isolates of F.
temperatum and F. subglutinans, either on stalk or ear. Differences in hybrids
responses and symptoms that isolates of fungal species caused on stalks and
ears were poorly visible. According to our knowledge, the F. temperaturm
occurrence on sorghum seed as well as its pathogenicity on maize ear and stalk
are detected for the first time in the world.
Keywords: F. temperatum, F. subglutinans, maize, sorghum
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