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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P41 - Evaluation of antifungal activity of ethanol and
methanol extracts from Punica granatum peels on
fungal strains producing mycotoxins
H. Kadi 1 , N. Nahal Bouderba 1 , S. Moghtet, H. A. Lazouni 2 .
1 Laboratoryof Plant Resource Development and Food Security in Semi Arid Areas, South West of
Algeria, BP 417, University of Bechar, Algeria; 2 Natural Product Laboratory, University Abou Bakr Belk
aid, BP119, ImamaTlemcen, Algeria
E-mail: hamidkadi08@yahoo.fr
The worldwide contamination of foods and feeds with mycotoxins poses a
significant health problem. Mycotoxins can cause acute or chronic intoxication
and damage to humans and animals after ingestion of contaminated food and
feed.
Considering these as a first step the objective of our work is to evaluate the
antifungal activity of some extracts from Punica granatum peels on some fungal
strains producing mycotoxins. Pomegranate is an ancient fruit and a known rich
source of bioactive compounds. It is only recently that modern scientists have
systematically evaluated the fruit for its various medicinally useful properties. In
the present work four extracts obtained by an ethanol, methanol extraction and
maceration from Punica granatum peels were tested for their antifungal potential
against three species of Aspergillus such as A. flavus, A. niger, A. ochraceus, and
one species of Penicillium such as P. expansum.
The assessment of antifungal activity is assayed by radial growth technique on
solid medium content selected volumes of peel Punicagranatum extracts. The
assessment of mycotoxinogenesis is released on Y.E.S medium mix with selected
volumes of peel Punicagranatum extracts. The results of our work indicate that all
the four extracts prevent totally the mycilial growth in solid and liquid medium with
a percentage of 100% of all the fungal strains in selected concentration. Moreover
the methanol extract was the more effective one and the fungal strain A.
ochraceus was the most sensible one for all the extracts.
In conclusion the results indicate that all the extracts which have been obtained
from Punica granatum peels got an antifungal activity against the four fungal
strains.
Keywords: mycotoxins, antifungal activity, Aspergillus, Penicillium, Punica
granatum peels, methanol extract, ethanol extract
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