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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY, BIOSYNTHESIS, FEED AND FOOD SAFETY P37 - Validation of an ELISA for the determination of fumonisin in maize samples for human consumption R. De Pace, C. Franchino, R. Damiano, M. Marino, V. Vita. Department of Chemistry, Experimental Zooprophylaxis Institute (IZS) of Puglia and Basilicata, Via Manfredonia 20, 71100, Italy E-mail: r.depace@libero.it The fumonisins are mycotoxins that mainly contaminate maize and its products. The enforcement of the recently established legal limit for fumonisins at the European Community level, as set out in Regulation CE/1126/2007, and the high incidence of contamination by these toxins of such a common grain crop as maize, have made the problem of the fumonisins even more immediate. The establishment of these maximum permissible fumonisin limits is thus justified both for health reasons and due to the need to provide the supervisory authorities and food companies with a precise normative reference. Following the indications of the European organisations relating to the analytical quality and reliability of data, it has become necessary to have a validated method for use in official monitoring that is both fast and reliable. The objective of this report is to provide the results of our study of a validation process for the fumonisins in maize used for human consumption that uses an enzyme-linked immunosorbent assay (ELISA), in agreement with European Community Decision 2002/657/EC. Based on the results obtained, the analytical method defined in our study achieves the required aim and represents a further tool that is now available to all operators in the sector of risk assessment. The applicability of this method was verified by performing tests on official samples obtained through the competent bodies. While this remains within the limits of a small case study, we can confirm that this monitoring system is effective, and that the levels of contamination found in the samples analysed do not raise concerns for health and hygiene at present. However, there remains the need to ensure continuity in the quality and quantity of the necessary actions for the protection of consumers from possible contamination by the fumonisins. Keywords: fumonisins, validation, analytical methods, exposure monitoring 129
SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY, BIOSYNTHESIS, FEED AND FOOD SAFETY P38 - Prediction of deoxynivalenol content in wheat by Near Infrared Reflectance Spectroscopy C. Tibola, J. M. C. Fernandes, M. Nicolau Brazilian Agricultural Research Corporation, Embrapa Wheat, Rodovia BR 285, km 294, 99001-970, Passo Fundo, RS, Brazil E-mail: casiane.tibola@embrapa.br The main Brazilian wheat growing area is located in the South, where the main limiting factor for wheat production is the excess of humidity, which favors Fusarium Head Blight (FHB) outbreaks. The mycotoxin analysis requires the development of rapid, reliable and sensitive screening methods, in order to meet legislation requirements and to protect consumers. Near infrared spectroscopy (NIRs) has been used as a method to predict the quality and safety of different agricultural products due to the speed of analysis, minimal sample preparation and low cost. The objectives were to conduct a large-scale and multi-year deoxynivalenol (DON) monitoring and to develop a NIRs calibration model based on DON results obtained by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 412 wheat kernel samples and 450 ground wheat samples from southern Brazilian wheat-growing regions, during the 2009 to 2011 cropping seasons, were analyzed. The wheat samples were scanned by NIRs before and after the milling process. Only the ground samples were analyzed for DON concentration by the reference method. The results from LC-MS/MS indicated that DON was abundant in the region and it was detected in 390 samples from 450 analyzed (86,6% of the total analyzed samples), in all period. The mean DON content was 1,516 µg kg -1 , ranging from 140 to 8,025 µg kg -1 . Cross-validation indicated that the calibration model was able to discriminate between low and high DON levels, for both whole wheat and ground wheat, explaining 71% and 83% of the variability, respectively. The NIRs technique is promising as a screening tool to segregate highly contaminated grain, thus providing safer products for feed and food industry. Furthermore, the technique may be useful to discard genotypes that accumulate high DON content in wheat breeding programs. Keywords: Triticum aestivum, Fusarium graminearum, screening methods, mycotoxin 130
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Bordeaux EFS12 12 th European Fusar
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TABLE OF CONTENTS Welcome from the
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4 SPONSORSHIP The 12 th European Fu
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8 13:00 - 14:00 Lunch 14:00 - 15:00
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10 Session 4: Genetics of Hosts - P
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12 Thursday, May 16 th Session 6: F
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Evidence for birth-and-death evolut
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Future challenges of Fusarium and m
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P35 - Screening deoxynivalenol in o
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P76 - Fusarium verticillioides and
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P113 - Agronomic practices and risk
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ORAL PRESENTATIONS 25
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KEYNOTE LECTURE SESSION 1: FUSARIUM
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SESSION 1: FUSARIUM - GENETICS, GEN
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SESSION 5: DISEASE CONTROL AND FORE
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de Hoog G. S., 89, 114, 236 de Kler
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Ponts N., 33, 36, 82, 94, 104, 106,
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BOKU-University of Natural Resource
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touatisihem03@yahoo.fr He Xinyao In
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steve.oliver@bioc.cam.ac. uk Ortega
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Subramaniam Gopal Agriculture and A
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