EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P35 - Screening deoxynivalenol in oat using a quickmethod
with comparison to a quantitative GC-MS
analysis
M. Rauvola 1 , T. Hovinen 1 , V. Hietaniemi 2 , J. Kaitaranta 1 , S. Rämö 2
1 Turku University of Applied Sciences, Lemminkäisenkatu 30, 20520 Turku, Finland and 2 MTT
Agrifood Research Finland, 31600 Jokioinen, Finland
E-mail: sari.ramo@mtt.fi
Fusarium appearance in grain depends on various aspects and in Finland oat is
the most sensitive to this contamination. The Commission Regulation (EC) No
1881/2006 sets maximum levels for mycotoxins in foodstuffs and their analysis is
increasingly conducted as a measure to grain approval for trade. These
quantitative measurements usually are time consuming and expensive and thus
new methods have been developed and are commercially available for the semi
quantitative quick analysis of specific compounds.
In this study the the Rida® Quick DON method with Rida® Quick Scan reader
were tested to the determination of deoxynivalenol (DON) in oat. The results were
compared to those with an accredited GC-MS analysis. For the interpretation of
the results the linear regression analysis was made and paired t test was applied
to reveal the 95 per cent significance level. An oat sample with the DON content
of 0.76 ppm (GC-MS analysis) resulted on average 0.81 ppm when analyzed in
six parallel samples with the RSD percentage of 14. The repeatability of the
analytical system itself in six determinations resulted in an average of 0.84 ppm
with RSD % 15. If the same sample was read for six times in the reader the
average value was 0.81 ppm with RSD % of only 2.4 %. Thereafter altogether 30
oat samples containing 0.5 – 2.6 ppm DON as measured by GC-MS were tested
followed by regression analysis. As a conclusion based on all these tests the semi
quantitative Rida® Quick DON met the requirements of the Commission
Regulation (EC) 401/2006 for a quantitative DON determination where the RSD
percentage should be equal or less than 20. The results, however, suggest that a
single analysis resulting the DON content close to legislative limits should be
confirmed with an accredited quantitative analysis.
Keywords: Fusarium, mycotoxins, DON, analysis
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