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EFS12- Book of abstracts - Contact

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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,<br />

BIOSYNTHESIS, FEED AND FOOD SAFETY<br />

P33 - Fusarium toxin in forage rice grown in a paddy<br />

field in Japan<br />

R. Uegaki, H. Inoue, M. Tohno, T. Tsukiboshi<br />

National Agriculture and Food Research Organization (NARO), Institute <strong>of</strong> Livestock and Grassland<br />

Science (NILGS), 768 Senbonmatsu, Nasushiobara, Tochigi, Japan<br />

E-mail: uegaki@affrc.go.jp<br />

The reduction, we investigated the occurrence <strong>of</strong> mycotoxin-producing Fusarium<br />

fungi on forage rice and the changes <strong>of</strong> fumonisin (FUM), deoxynivalenol<br />

(DON)nivalenol (NIV) and zearalenon (ZEN) in forage rice in Japan.<br />

Two rice cultivars, early maturing (A) and late maturing (B), were grown in a<br />

paddy field in Tochigi, Japan, 36.92°N, 139.93°E in 2011. The plants were<br />

sampled from heading stage to full-ripe stage. The Fusarium fungus on the plants<br />

was isolated and identified, and a qualified analysis <strong>of</strong> FUM, DON, NIV and ZEN<br />

in the plants was performed using an LC/MS/MS system.<br />

Eighteen Fusarium fungi were isolated from cultivar A, including 7 <strong>of</strong> F. fujikuroi<br />

(all FUM-producing), 5 <strong>of</strong> F. asiaticum (4 producing ZEN, 2 producing NIV and 2<br />

producing DON). Seven were isolated from cultivar B, including 4 <strong>of</strong> F. fujikuroi (all<br />

producing FUM) and 1 <strong>of</strong> F. graminearum (DON and ZEN-producing). These<br />

Fusarium fungi were isolated from the head but also from SL from the heading<br />

stage to the full-ripe stage in both cultivars. The concentration <strong>of</strong> FUM (as FUM<br />

B1) in the heads <strong>of</strong> cultivar A ranged from ND (

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