EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
EFS12- Book of abstracts - Contact
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SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,<br />
BIOSYNTHESIS, FEED AND FOOD SAFETY<br />
P31 - Quantification <strong>of</strong> Fusarium fungi and their<br />
mycotoxins in common buckwheat grain<br />
I. Kerienė, A. Mankevičienė, E. Bakšienė<br />
Institute <strong>of</strong> Agriculture, Lithuanian Research Centre for Agriculture and Forestry, LT-58344 Akademija,<br />
Kėdainiai district, Lithuania<br />
E-mail: ilona.keriene@gmail.com; audre@lzi.lt; eugenija.baksiene@voke.lzi.lt<br />
Harvested common buckwheat grains were assayed for the presence <strong>of</strong> Fusarium<br />
fungi and mycotoxins deoxynivalenol (DON), zearalenone (ZEA) and T2/HT2<br />
toxins at Institute <strong>of</strong> Agriculture, Lithuanian Research Centre for Agriculture and<br />
Forestry during 2011-2012. Buckwheat crops were grown under organic,<br />
sustainable and intensive cropping systems.<br />
The cd-ELISA method and Veratox Fast kits were used to identify and quantify<br />
mycotoxins, while Fusarium fungi species were identified using conventional fungi<br />
determination techniques. In 2011, the fungi <strong>of</strong> Fusarium genus were detected in<br />
12 % <strong>of</strong> the grain samples tested, while in 2012 in 75.7 % <strong>of</strong> the samples<br />
analysed. The following mycotoxin-producing species were identified: F.<br />
graminearum, F. equiseti, F. avenaceum, F. sporotrichioides, F. oxysporum, F.<br />
poae. In 2011, DON was detected in the range <strong>of</strong> 240.0 to 1010.2 µg kg -1 in 100%<br />
<strong>of</strong> the grain samples analysed. ZEA was detected in 25% <strong>of</strong> the samples tested.<br />
The established concentrations in most cases did not exceed the maximum<br />
allowable levels set forth by the EU regulation (EB) No. 1881/2006 for the grain<br />
intended for direct human consumption and for processed grain food products<br />
intended for infants and young children. Although the samples <strong>of</strong> grain harvested<br />
in 2012 were more heavily contaminated with Fusarium fungi compared with<br />
those harvested in 2011, DON was not detected at all and the concentrations <strong>of</strong><br />
ZEA and T2/HT2 toxin were very low. The fungi <strong>of</strong> F. avenaceum, F.<br />
sporotrichioides species that are not DON and ZEA producers, predominated in<br />
the grain samples.<br />
No effects <strong>of</strong> the cropping systems and crop rotations applied on the variation <strong>of</strong><br />
mycotoxins contents in common buckwheat seeds were revealed.<br />
Acknowledgements: The abstract presents research findings obtained through the<br />
long-term research programme “Harmful organisms in agro and forest<br />
ecosystems” implemented by Lithuanian Research Centre for Agriculture and<br />
Forestry.<br />
Keywords: common buckwheat, Fusarium fungi, mycotoxins<br />
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