EFS12- Book of abstracts - Contact

SESSION 2: SECONDARY METABOLITES – BIOCHEMISTRY,
BIOSYNTHESIS, FEED AND FOOD SAFETY
P31 - Quantification of Fusarium fungi and their
mycotoxins in common buckwheat grain
I. Kerienė, A. Mankevičienė, E. Bakšienė
Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry, LT-58344 Akademija,
Kėdainiai district, Lithuania
E-mail: ilona.keriene@gmail.com; audre@lzi.lt; eugenija.baksiene@voke.lzi.lt
Harvested common buckwheat grains were assayed for the presence of Fusarium
fungi and mycotoxins deoxynivalenol (DON), zearalenone (ZEA) and T2/HT2
toxins at Institute of Agriculture, Lithuanian Research Centre for Agriculture and
Forestry during 2011-2012. Buckwheat crops were grown under organic,
sustainable and intensive cropping systems.
The cd-ELISA method and Veratox Fast kits were used to identify and quantify
mycotoxins, while Fusarium fungi species were identified using conventional fungi
determination techniques. In 2011, the fungi of Fusarium genus were detected in
12 % of the grain samples tested, while in 2012 in 75.7 % of the samples
analysed. The following mycotoxin-producing species were identified: F.
graminearum, F. equiseti, F. avenaceum, F. sporotrichioides, F. oxysporum, F.
poae. In 2011, DON was detected in the range of 240.0 to 1010.2 µg kg -1 in 100%
of the grain samples analysed. ZEA was detected in 25% of the samples tested.
The established concentrations in most cases did not exceed the maximum
allowable levels set forth by the EU regulation (EB) No. 1881/2006 for the grain
intended for direct human consumption and for processed grain food products
intended for infants and young children. Although the samples of grain harvested
in 2012 were more heavily contaminated with Fusarium fungi compared with
those harvested in 2011, DON was not detected at all and the concentrations of
ZEA and T2/HT2 toxin were very low. The fungi of F. avenaceum, F.
sporotrichioides species that are not DON and ZEA producers, predominated in
the grain samples.
No effects of the cropping systems and crop rotations applied on the variation of
mycotoxins contents in common buckwheat seeds were revealed.
Acknowledgements: The abstract presents research findings obtained through the
long-term research programme “Harmful organisms in agro and forest
ecosystems” implemented by Lithuanian Research Centre for Agriculture and
Forestry.
Keywords: common buckwheat, Fusarium fungi, mycotoxins
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